Anti-Mitochondrial Calcium Uni

Anti-Mitochondrial Calcium Uniporter Regulator 1

MCUR1, Coiled-coil domain-containing protein 90A, mitochondrial, Ccdc90a
Cat #: ACC-321
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: WB
May also work in: IFC, IC, IP, IH
Reactivity: M, R

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
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Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse MCUR1. Anti-Mitochondrial Calcium Uniporter Regulator 1 antibody (#ACC-321) can be used in western blot analysis. It has been designed to recognize Mitochondrial Calcium Uniporter Regulator 1 from mouse and rat samples.

• Applications
• Specifications
• Scientific Background
• Related Products

Western blot

Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and rat skeletal muscle (lanes 3 and 6) lysates:
1-3. Anti-Mitochondrial Calcium Uniporter Regulator 1 antibody (#ACC-321), (1:200).
4-6. Anti-Mitochondrial Calcium Uniporter Regulator 1 antibody preincubated with the control peptide antigen.
Immunogen
Peptide (C)GRNALGRLRLGARR, corresponding to amino acid residues 97-110 of mouse Mitochondrial Calcium Uniporter Regulator 1 (Accession Q9CXD6). Intracellular loop, mitochondrial matrix.

HomologyRat - identical; human – no apparent homology.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.8 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

Ca²⁺ flux across the mitochondrial inner membrane regulates bioenergetics, cytoplasmic Ca²⁺ signals and activation of cell death pathways¹. Mitochondrial Ca²⁺ uptake occurs at regions of close apposition with intracellular Ca²⁺ release sites driven by the inner membrane voltage generated by oxidative phosphorylation and mediated by a Ca²⁺ selective ion channel called the uniporter^2,3. Mitochondrial calcium uniporter (MCU) was recently identified as an ion-conducting pore⁴. MCUR1 (mitochondrial calcium uniporter regulator 1), belongs to the CCDC90 family, an integral membrane protein required for MCU-dependent mitochondrial Ca²⁺ uptake. MCUR1 binds to MCU and regulates ruthenium-red-sensitive MCU-dependent Ca²⁺ uptake⁵.

MCUR1 contains two transmembrane helices, where the N- and the short C-termini are exposed to the inter-membrane space⁵.
MCUR1 is ubiquitously expressed in mammalian tissues including: skeletal muscle, brain, heart, lung, liver, kidney, large intenstine and spleen⁵.

It was found that cells depleted of MCUR1 have abnormal bioenergetic properties similar to those observed in cells in which Ca²⁺ uptake is blocked. Thus, MCUR1 is necessary for MCU to efficiently promote mitochondrial Ca²⁺ uptake and to ensure normal mitochondrial bioenergetics⁵.

References

1. Orrenius, S. et al. (2003) Nat. Rev. Mol. Cell Biol. 4, 552.
2. Csordas, G. et al. (2010) Mol. Cell 39, 121.
3. Santo-Domingo, J. and Demaurex, N. (2010) Biochim. Biophys. Acta 1797, 907.
4. Baughman, J.M. et al. (2011) Nature 476, 341.
5. Mallilankaraman, K. et al. (2012) Nat. Cell Biol. 14, 1336.