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- 详细信息
- 技术资料
- 库存:
大量
- 英文名:
pAP12BD
- 保质期:
1年
- 供应商:
再康生物
- 保存条件:
低温运输,-20℃保存
质粒编号:
质粒名称: pAP12BD
其他名称:
质粒大小(bp): 15000
抗性:
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性:
| Notes: |
The promoter and terminator sequences were derived from the phosphoglycerate kinase gene. Saccharomyces cerevisiae containing a single copy of the neomycin phosphotransferase gene are sensitive to G418; resistance to 400 ug/ml is expressed if there are several copies per cell. The G418-resistance segment includes a 0.7 kb region of the PGK promoter and a 1.5 kb BglII/BamHI fragment from Tn5. The BamHI site is between the G418R and the PGK terminator. Restriction digests of the clone give the following sizes (kb): BamHI–15.0; BglII–15.0; XhoI–9.4, 6.0; EcoRI–13.0, 2.1; KpnI–13.0, 2.2. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli JA221, Saccharomyces cerevisiae, E.coli.
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质粒图谱:
质粒序列:
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pAP12BD
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