Anti-Na+/H+ Exchanger 6 (NHE-6

Anti-Na⁺/H⁺ Exchanger 6 (NHE-6)

Sodium/Hydrogen Exchanger 6, NHE6, SLC9A6
Cat #: ANX-006
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: WB
May also work in: IC, IFC, IH, IP
Reactivity: H, M, R

Anti-Na⁺/H⁺ Exchanger 6 (NHE-6)Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
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Anti-Na⁺/H⁺ Exchanger 6 (NHE-6) (#ANX-006) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize NHE-6 from human, rat, and mouse samples.

• Applications
• Specifications
• Scientific Background
• Related Products

Western blot

Western blot analysis of rat brain membranes (lanes 1 and 3) and new born rat brain membranes (lanes 2 and 4):
1, 2. Anti-Na⁺/H⁺ Exchanger 6 (NHE-6) antibody (#ANX-006), (1:200).
3, 4. Anti-Na⁺/H⁺ Exchanger 6 (NHE-6) antibody, preincubated with the control peptide antigen.

Western blot analysis of mouse brain lysate:
1. Anti-Na⁺/H⁺ Exchanger 6 (NHE-6) antibody (#ANX-006), (1:200).
2. Anti-Na⁺/H⁺ Exchanger 6 (NHE-6) antibody, preincubated with the control peptide antigen.

Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
1. Anti-Na⁺/H⁺ Exchanger 6 (NHE-6) antibody (#ANX-006), (1:200).
2. Anti-Na⁺/H⁺ Exchanger 6 (NHE-6) antibody, preincubated with the control peptide antigen.
Immunogen
Peptide (C)EHLGVPENERRTTK, corresponding to amino acid residues 515 - 528 of human NHE-6 (Accession Q92581). Intracellular, C-terminus.

HomologyMouse, rat – 13/14 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.8 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

Intracellular pH regulation in the brain is extremely important in both physiological and pathophysiological conditions. Changes in Intracellular pH often result in altered neuronal excitability. In mammalian cells, intracellular pH against acidosis is generally regulated by ion transporters located on the plasma membrane called sodium-hydrogen exchangers (Na⁺/H⁺ exchanger, NHE)^1,2. In addition to their role in regulating pH, NHE transporters also function in a coupled mode to mediate recovery from decreased cell volume¹.

Several studies have suggested that Na⁺/H⁺ exchange is the predominant pH-regulatory mode in isolated nerve terminals. In addition, Na⁺/H⁺ exchange plays a role in regulating synaptic transmission at glutamatergic, GABAergic, and dopaminergic synapses¹.

The NHE family belongs to the Slc9 gene family of Na⁺ coupled transport proteins and comprises nine mammalian isoforms of Na⁺/H⁺ exchangers that are localized to the plasma membrane, NHE1-9 encoded by the SLC9A1-SLC9A9 genes, respectively. SLC9 family members have a short N terminus and an extensive C terminus that plays a regulatory role².

NHE6 is localized to early and recycling endosomes, where it exchanges luminal H⁺ for Na⁺ or K⁺. Its expression is highest in the spinal cord and brain and is particularly enriched in the hippocampus, cortex, and Purkinje cell layer of the cerebellum³. Mutations in NHE6 encoding gene have been linked to autism, Christianson syndrome, and Angelman-like syndrome.

References

1. Siyanov, V. and Baltz, J.M. (2013) Biol. Reprod. 88, 157.
2. Ruffin, V.A. et al. (2014) Front. Physiol. 5, 43.
3. Prasad, H. and Rao, R. (2015) J. Biol. Chem. 290, 5311.