Anti-Glucose Transporter 3 (GL

Anti-Glucose Transporter 3 (GLUT3) (extracellular)

Facilitated glucose transporter member 3, SLC2A3
Cat #: AGT-023
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: IFC, IH, WB
May also work in: IC, IP, LCI
Reactivity: M, R

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Anti-Glucose Transporter 3 (GLUT3) (extracellular)Species reactivity key:

H- Human, M- Mouse, R- Rat
CLICK HERE TO RECEIVE A 25 µl FREE TRIAL SAMPLE!

Anti-Glucose Transporter 3 (GLUT3) (extracellular) (#AGT-023) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. The antibody has been designed to recognize GLUT3 from rat and mouse samples. It will not recognize GLUT3 from human samples.

• Applications
• Specifications
• Scientific Background
• Related Products

Western blot

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
1, 2. Anti-Glucose Transporter 3 (GLUT3) (extracellular) antibody (#AGT-023), (1:400).
3, 4. Anti-Glucose Transporter 3 (GLUT3) (extracellular) antibody, preincubated with the control peptide antigen.
Immunohistochemistry

Expression of GLUT3 in mouse hippocampus and cerebellum
Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Glucose Transporter 3 (GLUT3) (extracellular) antibody (#AGT-023), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. GLUT3 staining (green) in mouse hippocampal dentate gyrus, is detected in interneurons (arrows pointing up) in the Hilus and granule layer (G) (arrow pointing down). B. Staining in mouse cerebellum, is seen in purkinje cells (vertical arrows) and dendrites (horizontal arrows) in the molecular layer (Mol). Cell nuclei were stained with DAPI (blue).
Indirect flow cytometry

Indirect flow cytometry of live intact mouse J774 macrophage cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-Glucose Transporter 3 (GLUT3) (extracellular) antibody (#AGT-023), (5 µg) + goat-anti-rabbit-FITC.
Immunogen
Peptide (C)KDFLNYTLEERLED, corresponding to amino acid residues 39-52 of rat Glucose Transporter 3 (Accession Q07647). 1^st extracellular loop.

HomologyMouse – identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
SpecificityWill not recognize human GLUT3.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.8 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

The GLUT protein family is encoded by the SLC2 genes and are a member of the major facilitator superfamily of membrane transporters. GLUTs are recognized as key regulators of whole-body glucose homeostasis, and are responsible for the uptake of carbohydrate-derived glucose by cells. There are 14 GLUT isoforms divided into 3 different group classes based on sequence similarity and structural and functional characteristics^1,2.

GLUT3 is a low-K_m protein responsible for glucose uptake into neurons. It is predominantly expressed in neurons and is commonly called “neuronal glucose transporter". The protein contains 12 membrane-spanning domains, an N-linked glycosylation site in loop 1 responsible for high-affinity transport of glucose, and intracellular NH₂- and COOH- termini. In addition, there are several conserved residues and motifs designated “sugar transporter signatures”^1,2.

GLUT3 levels are high in some malignant glioma cells compared to normal glial cells and therefore may be used as a therapeutic target in some cancer treatment³.

References

1. Lacombe, V.A. (2014) ISRN Vet. Sci. 2014, 409547.
2. Simpson, I.A. et al. (2024) Am J. Physiol. 295, E242.
3. Zheng, C. et al. (2016) Oncol. Lett. 12, 125.