Anti-CaV1.1 (extracellular)

Anti-Ca_V1.1 (extracellular)

Voltage-dependent L-type calcium channel subunit alpha-1S, Cach1, Cach1b, Cacn1, Cacnl1a3, Cacna1s
Cat #: ACC-314
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: IH, WB
May also work in: IFC, IC, LCI, IP
Reactivity: H, M, R

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
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Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat Ca_V1.1. Anti-Ca_V1.1 (extracellular) antibody (#ACC-314) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the channel in living cells. It has been designed to recognize Ca_V1.1 from rat, mouse and human samples.
 

• Applications
• Specifications
• Scientific Background
• Related Products

Western blot

Western blot analysis of rat skeletal muscle:
1. Anti-Ca_V1.1 (extracellular) antibody (#ACC-314), (1:200).
2. Anti-Ca_V1.1 (extracellular) antibody preincubated with the control peptide antigen.
Immunohistochemistry

Expression of Ca_V1.1 in rat skeletal muscle
Immunohistochemical staining of paraffin-embedded rat skeletal muscle sections using Anti-Ca_V1.1 (extracellular) antibody (#ACC-314), (1:100), followed by DAB. Ca_V1.1 staining is highly specific for striated muscle cells. Note that smooth muscle cells of arteries are not stained (arrow).  Hematoxilin is used as the counterstain.
Immunogen
Peptide CGNVDPDESARIS, corresponding to amino acid residues 506-518 of rat Ca_V1.1 (Accession Q02485). 2^nd extracellular loop, domain IV.

HomologyMouse, human - identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.85 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

Voltage-gated Ca²⁺ channels (VGCCs) serve as key transducers coupling changes in cell surface membrane potential with local intracellular Ca²⁺ pathways. Among the three families of VGCCs, L-type Ca²⁺ channels (L-VGCCs), include four different isoforms of the α1 pore-forming subunit: Ca_V1.1, Ca_V1.2, Ca_V1.3 and Ca_V1.4¹.

Ca_V1.1 (α1S) is the prototypical VGCCs. It was the first member of the Ca_V family to be cloned². Ca_V1.1 is a single polypeptide composed of four relatively conserved repeats (I, II, II and IV) containing six α-helices a piece. The fourth α-helix of each repeat has a regularly spaced sequence of basic residues that is believed to be critical for voltage-sensing. The I–II-linker is the site of interaction with the intracellular β1a subunit ³.

Ca_V1.1 is expressed solely in skeletal muscle. It is localized in regions of the T-tubular membrane that are closely opposed to the sarcoplasmic reticulum (the TSR junction).  The primary role of Ca_V1.1 is to serve as the voltage sensor for skeletal muscle-type excitation contraction (EC) coupling⁴.

Mutations in Ca_V1.1 have been identified as causative for two congenital muscle pathophysiologies: hypokalemic periodic paralysis⁵ (HypoKPP) and malignant hyperthermia⁶.

References

1. Zuccotti, A. et al. (2011) Trends Pharmacol. Sci. 32, 366.
2. Tanabe, T. et al. (1987) Nature 328, 313.
3. Bannister, R.A. and Beam, K.G. (2013) Biochim. Biophys. Acta 1828, 1587.
4. Stroffekova, K. (2024) Pflugers Arch. 455, 873.
5. Renner, D.R. and Ptacek, L.J. (2002) Adv. Neurol. 88, 235.
6. Jurkat-Rott, K. Lehmann-Horn, F. (2005) J. Clin. Invest. 115, 2000.