- $247
- Alomone
- ZK-0039467
- 以色列
- 2025年11月20日
- WB
- Rabbit
- 见官方网站
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海再康生物科技有限公司
- 库存：
大量
- 靶点：
见官方网站
- 级别：
高
- 目录编号：
ACS-003
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
6个月
- 抗体英文名：
Anti-Calcium-Binding Protein 2
- 抗体名：
Anti-Calcium-Binding Protein 2
- 标记物：
见官方网站
- 宿主：
Rabbit
- 适应物种：
见官方网站
- 免疫原：
见官方网站
- 亚型：
见官方网站
- 形态：
液体或冻干粉
- 应用范围：
WB
- 浓度：
见官方网站
- 保存条件：
-20°C
- 规格：
25 µl, 50 µl, 0.2 ml
Anti-Calcium-Binding Protein 2
Cabp2Cat #: ACS-003
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: WB
May also work in: IC, IH, IP
Reactivity: H, M, R
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blotSpecies reactivity key:
H- Human, M- Mouse, R- RatCLICK HERE TO RECEIVE A 25 µl FREE TRIAL SAMPLE!
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse CaBP2. Anti-Calcium-Binding Protein 2 antibody (#ACS-003) can be used in western blot analysis. It has been designed to recognize Calcium-binding protein 2 from rat, mouse and human samples.
- Applications
- Specifications
- Scientific Background
- Related Products
Western blot analysis of ARPE-19 (lanes 1 and 4), mouse heart (lanes 2 and 5) and rat heart (lanes 3 and 6) lysates:
1-3. Anti-Calcium-Binding Protein 2 antibody (1:400), (#ACS-003)
4-6. Anti-Calcium-Binding Protein 2 antibody preincubated with the control peptide antigen.
Immunogen
Peptide CAKTPWH RGSKER, corresponding to amino acid residues 4-16 of mouse Calcium-Binding Protein 2 (Accession Q9JLK4). Intracellular, N-terminus.
HomologyRat – 12/13 amino acid residues identical; human – 9/13 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.85 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background
Neuronal Ca2+-binding proteins (CaBP1-5) are a subclass of the calmodulin (CaM) superfamily that regulates specific Ca2+ channel targets in the brain and retina. Multiple isoforms of CaBPs are localized in different neuronal cell types and perform specialized roles in sensory transduction and disease processes1.
CaBP1–5 proteins have four EF hands that form pairs within the N lobe (EF1 and 2) and C lobe (EF3 and 4). The two lobes are structurally independent and connected by a flexible linker. Whereas all four EF hands bind Ca2+ in CaM, EF2 in CaBP1 does not bind Ca2+, and EF1 has reduced selectivity for Ca2+ over Mg2+. EF3 and EF4 in the C lobe of CaBP1 exhibit canonical Ca2+-induced conformational changes2. CaBP-target interactions induce functional changes distinct from those caused by CaM and may diversify neuronal responses to Ca2+ signals. CaBPs interact with and reshape the functional properties of certain voltage-gated Ca2+ channels (CaVs)3.
CaBP1 is the best characterized family member and has been shown to regulate inositol 1,4,5-triphosphate receptors (IP3Rs), P/Q-type voltage-gated Ca2+ channels, L-type Ca2+ channels, and the transient receptor potential channel (TRPC5)4. CaBP1 regulates the currents of CaV1 and CaV2 channels in neurons, photoreceptors, and auditory hair cells. CaBP interactions with CaV1 channels may be required for hearing and vision, as mutations that disrupt these interactions cause blindness and deafness5.
References
- Park, S. et al. (2011) Protein Sci. 20, 1356.
- Li, C. et al. (2009) J. Biol. Chem. 284, 2472.
- Findeisen, F. et al. (2010) Structure 18, 1617.
- Kinoshita-Kawada, M. et al. (2005) Pflugers Arch. 450, 345.
- Schrauwen, I. et al. (2012) Am. J. Hum. Genet. 91, 636.
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文献和实验广义的是指与钙离子进行特异结合的蛋白质之总称。与活体内钙的多种功能相应地有多种功能的蛋白质，可分别为以Ca 2 为活化因子的酶（血液凝固因子、各种蛋白酶、磷脂酶 A 2 和α - 淀粉酶等分解酶、膜的 Ca 2 运输 ATPase 、磷酸化酶 b 激酶）及无酶活性的两类，后者多称为狭义的钙结合蛋白，其中又分为：（ 1 ）与酶或细胞的机能调控有关的；（ 2 ）通过与 Ca 2 结合使游离的离子浓度降低，具有贮藏 Ca 的功能的。但也有功能未被测定的。肌宁蛋白 C 及调钙蛋白
Characterization of Calcium Channel Binding
calcium channels are expressed in a variety of tissues including heart, muscles and brain. Saturation binding of a radioligand to the calcium channel is commonly used to characterize the expression level of the channel protein. Compound competition binding
was obtained when using an anti-RGS-(His)4 antibody. Thus in a sandwich binding assay the anti-RGS-(His)4 antibody can be utilized as an appropriate tool for stable yet reversible capturing of RGS-(His)6-tagged proteins with a non-His-tagged protein.
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