Anti-CaVγ7 (extracellular)

Anti-Ca_Vγ7 (extracellular)

Voltage-dependent calcium channel gamma-7 subunit, Transmembrane AMPAR regulatory protein gamma-7, TARP gamma-7, CACNG7
Cat #: ACC-109
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: WB
May also work in: IC, IFC, IH, IP, LCI
Reactivity: H, M, R

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
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Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Ca_Vγ7. Anti-Ca_Vγ7 antibody (#ACC-109) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting Ca_Vγ7 in living cells. It has been designed to recognize Ca_Vγ7 from rat, mouse and human samples.

• Applications
• Specifications
• Scientific Background
• Related Products

Western blot

Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
1, 2. Anti-Ca_Vγ7 (extracellular) antibody (#ACC-109), (1:1000).
3, 4. Anti-Ca_Vγ7 (extracellular) antibody preincubated with the control peptide antigen.
Immunogen
Peptide (C)REKGR(S)VASEYFLE, corresponding to amino acid residues 67-80 of human Ca_Vγ7 (Accession P62955). 1^st extracellular loop.

HomologyRat, mouse –13/14 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.85 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

Transmembrane AMPA receptor regulatory proteins (TARPs) serve as auxiliary subunits of AMPA receptors and regulate functional aspects of these receptors such as: fast excitatory synaptic transmission, surface trafficking, synaptic clustering and glutamate affinity. Generally, TARPs are responsible for regulating expression, channel properties and localization of AMPA receptors in the brain¹.

TARPs are non-pore-forming integral membrane proteins with four transmembrane domains that are widely expressed in the CNS. TARP family is divided in two: Type I and type II. Type I TARPs include two calcium channel γ subunits: γ5 and γ7, also known as Cacng5 and Cacng7, respectively.They do not traffic AMPAR, they only modulate existing AMPAR channel function².

Ca_Vγ7 (Transmembrane AMPA Receptor Regulatory Protein Isoform γ7) is highly expressed in Purkinje cells of the cerebellum and in glomerular synapses in the granule cell layer of the cerebellum and significantly enhances glutamate-evoked currents and modulates gating of AMPARs. Ca_Vγ7 appears to derive from cerebellar Golgi cells^2,3.                       

Ca_Vγ7 shows the largest sequence divergence compared to other TARP members. Its structure demonstrates a short C-terminus with an absence of the TTPV motif that is critical in the binding of TARP-associated AMPARs to PSD-95 and in clustering at the synapse³.

Several recent studies have suggested that Ca_Vγ7 has the ability to associate with extrasynaptic calcium-permeable AMPA receptors and plays an important role in regulating the presence of neuronal calcium-permeable AMPA receptors at synapses³. In addition, transfection of TARP Ca_Vγ7 causes AMPA receptors to resensitize upon continued glutamate application³.

References

1. Cho, C.H. et al. (2007) Neuron. 55, 890.
2. Hamad, M.I. et al. (2014) Development. 141, 1737.
3. Studniarczyk, D. et al. (2013) Nat. Neurosci. 16, 1266.