Anti-Pneumolysin

Anti-Pneumolysin

Cat. No.

HYB 041-01

Product name

Anti-Pneumolysin

Description

Mouse monoclonal antibody

Specificity

HYB 041-01 reacts specifically with Pneumolysin from at least 9 different types of pneumococcal strains when tested by SDS-PAGE and WB. The tested pneumococcal types were 5, 6A, 11A, 11F, 12B, 15F, 19A, 25F and 35A.

Immunogen

Pneumolysin

Application

ELISA, WB

Species reactivity (positive)

S. pneumoniae

Species reactivity (negative)

Not determined

Subclass

IgG1/k

Clone number

1F11

Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Pneumolysin (pneumococcal hemolysin) is an intracellular protein produced by all strains of S. pneumoniae and is a cytoplasmic cholesterol-dependent cytolysin forming large pores in cholesterol-containing membranes and are therefore cytotoxic to mammalian cells. The molecular weight of Pneumolysin is approximately 53 kD