- $410
- 丹麦BioPorto Diagnostics A/S
- ZK-0039257
- 丹麦
- 2025年11月20日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 保质期：
12个月
- 供应商：
上海再康生物科技有限公司
- 保存条件：
4-8ºC without exposure to light. No precautions necessary during handling.
- 规格：
200ug/1mg
Anti-Alkaline phosphatase (Bovine)
- Cat. No.
- HYB 252-08
- Product name
- Anti-Alkaline phosphatase (Bovine)
- Description
-
Mouse monoclonal antibody
- Specificity
-
Alkaline phosphatase
- Immunogen
-
Alkaline phosphatase from calf intestine
- Application
- ELISA
- Species reactivity (positive)
- Bovine
- Species reactivity (negative)
- Not determined
- Subclass
-
IgG1/k
- Gene ID
- 280993
- Clone number
- 3F11
- Epitope
-
Not determined
- Content
-
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
-
Alkaline phosphatatase is a hydrolase enzyme responsible for removing phosphate groups (dephosphorylation) from many types of molecules, including nucleotides, proteins, and alkaloids.
Alkaline phosphatase is made in the liver, bone, and the placenta and normally present in high concentrations in growing bone and in bile.
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文献和实验Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48)
in solution c. for 5-15 minutes at room temperature. Wash, counterstain in haematoxylin, wash. Mount in phosphate buffered glycerine jelly RESULTS Alkaline phosphatase activity - Red Nuclei - Blue
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity 1. Obtain embryo samples, tube of AP substrate buffer and tube of phosphate buffered saline ( PBS ) for each group. Allow embryos
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