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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
pET-11b
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号: 60908-2774b
质粒名称: pET-11b
其他名称: pET 11b
质粒大小（bp）: 5676
抗性: Ampicillin
质粒类型: 大肠杆菌表达载体
是否病毒: 非病毒
启动子: T7/lacO
稳转或瞬转: 瞬时表达
组成型或诱导性: 组成型
表达水平: 高
蛋白标签:N-T7
5’测序引物: T7:5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性: Approximate size is 5675 bp. Hosts: E.coli. Related vectors: pBR322.
质粒图谱:
质粒序列:
| 1 | ttctcatgtt | tgacagctta | tcatcgataa | gctttaatgc | ggtagtttat | 50 |
| 51 | cacagttaaa | ttgctaacgc | agtcaggcac | cgtgtatgaa | atctaacaat | 100 |
| 101 | gcgctcatcg | tcatcctcgg | caccgtcacc | ctggatgctg | taggcatagg | 150 |
| 151 | cttggttatg | ccggtactgc | cgggcctctt | gcgggatatc | cggatatagt | 200 |
| 201 | tcctcctttc | agcaaaaaac | ccctcaagac | ccgtttagag | gccccaaggg | 250 |
| 251 | gttatgctag | ttattgctca | gcggtggcag | cagccaactc | agcttccttt | 300 |
| 301 | cgggctttgt | tagcagccgg | atcccgaccc | atttgctgtc | caccagtcat | 350 |
| 351 | gctagccata | tgtatatctc | cttcttaaag | ttaaacaaaa | ttatttctag | 400 |
| 401 | aggggaattg | ttatccgctc | acaattcccc | tatagtgagt | cgtattaatt | 450 |
| 451 | tcgcgggatc | gagatctcga | tcctctacgc | cggacgcatc | gtggccggca | 500 |
| 501 | tcaccggcgc | cacaggtgcg | gttgctggcg | cctatatcgc | cgacatcacc | 550 |
| 551 | gatggggaag | atcgggctcg | ccacttcggg | ctcatgagcg | cttgtttcgg | 600 |
| 601 | cgtgggtatg | gtggcaggcc | ccgtggccgg | gggactgttg | ggcgccatct | 650 |
| 651 | ccttgcatgc | accattcctt | gcggcggcgg | tgctcaacgg | cctcaaccta | 700 |
| 701 | ctactgggct | gcttcctaat | gcaggagtcg | cataagggag | agcgtcgaga | 750 |
| 751 | tcccggacac | catcgaatgg | cgcaaaacct | ttcgcggtat | ggcatgatag | 800 |
| 801 | cgcccggaag | agagtcaatt | cagggtggtg | aatgtgaaac | cagtaacgtt | 850 |
| 851 | atacgatgtc | gcagagtatg | ccggtgtctc | ttatcagacc | gtttcccgcg | 900 |
| 901 | tggtgaacca | ggccagccac | gtttctgcga | aaacgcggga | aaaagtggaa | 950 |
| 951 | gcggcgatgg | cggagctgaa | ttacattccc | aaccgcgtgg | cacaacaact | 1000 |
| 1001 | ggcgggcaaa | cagtcgttgc | tgattggcgt | tgccacctcc | agtctggccc | 1050 |
| 1051 | tgcacgcgcc | gtcgcaaatt | gtcgcggcga | ttaaatctcg | cgccgatcaa | 1100 |
| 1101 | ctgggtgcca | gcgtggtggt | gtcgatggta | gaacgaagcg | gcgtcgaagc | 1150 |
| 1151 | ctgtaaagcg | gcggtgcaca | atcttctcgc | gcaacgcgtc | agtgggctga | 1200 |
| 1201 | tcattaacta | tccgctggat | gaccaggatg | ccattgctgt | ggaagctgcc | 1250 |
| 1251 | tgcactaatg | ttccggcgtt | atttcttgat | gtctctgacc | agacacccat | 1300 |
| 1301 | caacagtatt | attttctccc | atgaagacgg | tacgcgactg | ggcgtggagc | 1350 |
| 1351 | atctggtcgc | attgggtcac | cagcaaatcg | cgctgttagc | gggcccatta | 1400 |
| 1401 | agttctgtct | cggcgcgtct | gcgtctggct | ggctggcata | aatatctcac | 1450 |
| 1451 | tcgcaatcaa | attcagccga | tagcggaacg | ggaaggcgac | tggagtgcca | 1500 |
| 1501 | tgtccggttt | tcaacaaacc | atgcaaatgc | tgaatgaggg | catcgttccc | 1550 |
| 1551 | actgcgatgc | tggttgccaa | cgatcagatg | gcgctgggcg | caatgcgcgc | 1600 |
| 1601 | cattaccgag | tccgggctgc | gcgttggtgc | ggatatctcg | gtagtgggat | 1650 |
| 1651 | acgacgatac | cgaagacagc | tcatgttata | tcccgccgtt | aaccaccatc | 1700 |
| 1701 | aaacaggatt | ttcgcctgct | ggggcaaacc | agcgtggacc | gcttgctgca | 1750 |
| 1751 | actctctcag | ggccaggcgg | tgaagggcaa | tcagctgttg | cccgtctcac | 1800 |
| 1801 | tggtgaaaag | aaaaaccacc | ctggcgccca | atacgcaaac | cgcctctccc | 1850 |
| 1851 | cgcgcgttgg | ccgattcatt | aatgcagctg | gcacgacagg | tttcccgact | 1900 |
| 1901 | ggaaagcggg | cagtgagcgc | aacgcaatta | atgtaagtta | gctcactcat | 1950 |
| 1951 | taggcaccgg | gatctcgacc | gatgcccttg | agagccttca | acccagtcag | 2000 |
| 2001 | ctccttccgg | tgggcgcggg | gcatgactat | cgtcgccgca | cttatgactg | 2050 |
| 2051 | tcttctttat | catgcaactc | gtaggacagg | tgccggcagc | gctctgggtc | 2100 |
| 2101 | attttcggcg | aggaccgctt | tcgctggagc | gcgacgatga | tcggcctgtc | 2150 |
| 2151 | gcttgcggta | ttcggaatct | tgcacgccct | cgctcaagcc | ttcgtcactg | 2200 |
| 2201 | gtcccgccac | caaacgtttc | ggcgagaagc | aggccattat | cgccggcatg | 2250 |
| 2251 | gcggccgacg | cgctgggcta | cgtcttgctg | gcgttcgcga | cgcgaggctg | 2300 |
| 2301 | gatggccttc | cccattatga | ttcttctcgc | ttccggcggc | atcgggatgc | 2350 |
| 2351 | ccgcgttgca | ggccatgctg | tccaggcagg | tagatgacga | ccatcaggga | 2400 |
| 2401 | cagcttcaag | gatcgctcgc | ggctcttacc | agcctaactt | cgatcactgg | 2450 |
| 2451 | accgctgatc | gtcacggcga | tttatgccgc | ctcggcgagc | acatggaacg | 2500 |
| 2501 | ggttggcatg | gattgtaggc | gccgccctat | accttgtctg | cctccccgcg | 2550 |
| 2551 | ttgcgtcgcg | gtgcatggag | ccgggccacc | tcgacctgaa | tggaagccgg | 2600 |
| 2601 | cggcacctcg | ctaacggatt | caccactcca | agaattggag | ccaatcaatt | 2650 |
| 2651 | cttgcggaga | actgtgaatg | cgcaaaccaa | cccttggcag | aacatatcca | 2700 |
| 2701 | tcgcgtccgc | catctccagc | agccgcacgc | ggcgcatctc | gggcagcgtt | 2750 |
| 2751 | gggtcctggc | cacgggtgcg | catgatcgtg | ctcctgtcgt | tgaggacccg | 2800 |
| 2801 | gctaggctgg | cggggttgcc | ttactggtta | gcagaatgaa | tcaccgatac | 2850 |
| 2851 | gcgagcgaac | gtgaagcgac | tgctgctgca | aaacgtctgc | gacctgagca | 2900 |
| 2901 | acaacatgaa | tggtcttcgg | tttccgtgtt | tcgtaaagtc | tggaaacgcg | 2950 |
| 2951 | gaagtcagcg | ccctgcacca | ttatgttccg | gatctgcatc | gcaggatgct | 3000 |
| 3001 | gctggctacc | ctgtggaaca | cctacatctg | tattaacgaa | gcgctggcat | 3050 |
| 3051 | tgaccctgag | tgatttttct | ctggtcccgc | cgcatccata | ccgccagttg | 3100 |
| 3101 | tttaccctca | caacgttcca | gtaaccgggc | atgttcatca | tcagtaaccc | 3150 |
| 3151 | gtatcgtgag | catcctctct | cgtttcatcg | gtatcattac | ccccatgaac | 3200 |
| 3201 | agaaatcccc | cttacacgga | ggcatcagtg | accaaacagg | aaaaaaccgc | 3250 |
| 3251 | ccttaacatg | gcccgcttta | tcagaagcca | gacattaacg | cttctggaga | 3300 |
| 3301 | aactcaacga | gctggacgcg | gatgaacagg | cagacatctg | tgaatcgctt | 3350 |
| 3351 | cacgaccacg | ctgatgagct | ttaccgcagc | tgcctcgcgc | gtttcggtga | 3400 |
| 3401 | tgacggtgaa | aacctctgac | acatgcagct | cccggagacg | gtcacagctt | 3450 |
| 3451 | gtctgtaagc | ggatgccggg | agcagacaag | cccgtcaggg | cgcgtcagcg | 3500 |
| 3501 | ggtgttggcg | ggtgtcgggg | cgcagccatg | acccagtcac | gtagcgatag | 3550 |
| 3551 | cggagtgtat | actggcttaa | ctatgcggca | tcagagcaga | ttgtactgag | 3600 |
| 3601 | agtgcaccat | atatgcggtg | tgaaataccg | cacagatgcg | taaggagaaa | 3650 |
| 3651 | ataccgcatc | aggcgctctt | ccgcttcctc | gctcactgac | tcgctgcgct | 3700 |
| 3701 | cggtcgttcg | gctgcggcga | gcggtatcag | ctcactcaaa | ggcggtaata | 3750 |
| 3751 | cggttatcca | cagaatcagg | ggataacgca | ggaaagaaca | tgtgagcaaa | 3800 |
| 3801 | aggccagcaa | aaggccagga | accgtaaaaa | ggccgcgttg | ctggcgtttt | 3850 |
| 3851 | tccataggct | ccgcccccct | gacgagcatc | acaaaaatcg | acgctcaagt | 3900 |
| 3901 | cagaggtggc | gaaacccgac | aggactataa | agataccagg | cgtttccccc | 3950 |
| 3951 | tggaagctcc | ctcgtgcgct | ctcctgttcc | gaccctgccg | cttaccggat | 4000 |
| 4001 | acctgtccgc | ctttctccct | tcgggaagcg | tggcgctttc | tcatagctca | 4050 |
| 4051 | cgctgtaggt | atctcagttc | ggtgtaggtc | gttcgctcca | agctgggctg | 4100 |
| 4101 | tgtgcacgaa | ccccccgttc | agcccgaccg | ctgcgcctta | tccggtaact | 4150 |
| 4151 | atcgtcttga | gtccaacccg | gtaagacacg | acttatcgcc | actggcagca | 4200 |
| 4201 | gccactggta | acaggattag | cagagcgagg | tatgtaggcg | gtgctacaga | 4250 |
| 4251 | gttcttgaag | tggtggccta | actacggcta | cactagaagg | acagtatttg | 4300 |
| 4301 | gtatctgcgc | tctgctgaag | ccagttacct | tcggaaaaag | agttggtagc | 4350 |
| 4351 | tcttgatccg | gcaaacaaac | caccgctggt | agcggtggtt | tttttgtttg | 4400 |
| 4401 | caagcagcag | attacgcgca | gaaaaaaagg | atctcaagaa | gatcctttga | 4450 |
| 4451 | tcttttctac | ggggtctgac | gctcagtgga | acgaaaactc | acgttaaggg | 4500 |
| 4501 | attttggtca | tgagattatc | aaaaaggatc | ttcacctaga | tccttttaaa | 4550 |
| 4551 | ttaaaaatga | agttttaaat | caatctaaag | tatatatgag | taaacttggt | 4600 |
| 4601 | ctgacagtta | ccaatgctta | atcagtgagg | cacctatctc | agcgatctgt | 4650 |
| 4651 | ctatttcgtt | catccatagt | tgcctgactc | cccgtcgtgt | agataactac | 4700 |
| 4701 | gatacgggag | ggcttaccat | ctggccccag | tgctgcaatg | ataccgcgag | 4750 |
| 4751 | acccacgctc | accggctcca | gatttatcag | caataaacca | gccagccgga | 4800 |
| 4801 | agggccgagc | gcagaagtgg | tcctgcaact | ttatccgcct | ccatccagtc | 4850 |
| 4851 | tattaattgt | tgccgggaag | ctagagtaag | tagttcgcca | gttaatagtt | 4900 |
| 4901 | tgcgcaacgt | tgttgccatt | gctgcaggca | tcgtggtgtc | acgctcgtcg | 4950 |
| 4951 | tttggtatgg | cttcattcag | ctccggttcc | caacgatcaa | ggcgagttac | 5000 |
| 5001 | atgatccccc | atgttgtgca | aaaaagcggt | tagctccttc | ggtcctccga | 5050 |
| 5051 | tcgttgtcag | aagtaagttg | gccgcagtgt | tatcactcat | ggttatggca | 5100 |
| 5101 | gcactgcata | attctcttac | tgtcatgcca | tccgtaagat | gcttttctgt | 5150 |
| 5151 | gactggtgag | tactcaacca | agtcattctg | agaatagtgt | atgcggcgac | 5200 |
| 5201 | cgagttgctc | ttgcccggcg | tcaacacggg | ataataccgc | gccacatagc | 5250 |
| 5251 | agaactttaa | aagtgctcat | cattggaaaa | cgttcttcgg | ggcgaaaact | 5300 |
| 5301 | ctcaaggatc | ttaccgctgt | tgagatccag | ttcgatgtaa | cccactcgtg | 5350 |
| 5351 | cacccaactg | atcttcagca | tcttttactt | tcaccagcgt | ttctgggtga | 5400 |
| 5401 | gcaaaaacag | gaaggcaaaa | tgccgcaaaa | aagggaataa | gggcgacacg | 5450 |
| 5451 | gaaatgttga | atactcatac | tcttcctttt | tcaatattat | tgaagcattt | 5500 |
| 5501 | atcagggtta | ttgtctcatg | agcggataca | tatttgaatg | tatttagaaa | 5550 |
| 5551 | aataaacaaa | taggggttcc | gcgcacattt | ccccgaaaag | tgccacctga | 5600 |
| 5601 | cgtctaagaa | accattatta | tcatgacatt | aacctataaa | aataggcgta | 5650 |
| 5651 | tcacgaggcc | ctttcgtctt | caagaa | 5676 |
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pET-11b
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