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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
pET-14b
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号: 60908-2776y
质粒名称: pET-14b
其他名称: pET 14b
质粒大小（bp）: 4671
抗性: Ampicillin (氨苄 青 霉 素)
质粒类型: 大肠杆菌表达载体
是否病毒: 非病毒
启动子: T7
稳转或瞬转: 瞬时表达
组成型或诱导性: 组成型
表达水平: 高
蛋白标签: N-His, N-thrombin
5′ 测序引物: T7
5′ 测序引物序列: 5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性: N term thrombin cleavage site; no lac.
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| tet (w/ gaps) | 86 | 1584 | |
| T7_terminator | 507 | 379 | |
| T7_Terminal_primer | 447 | 465 | |
| T7_transl_en_RBS | 607 | 591 | |
| T7_promoter | 662 | 644 | |
| pBRrevBam_primer | 765 | 746 | |
| ROP | 2223 | 2414 | |
| pGEX_3_primer | 2430 | 2408 | |
| Ampicillin | 4463 | 3603 | |
| AmpR_promoter | 4533 | 4505 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 1 | 799 | 1584 | |
| ORF frame 1 | 1389 | 931 | |
| ORF frame 2 | 4463 | 3603 |
| Enzyme Name | Cut |
|---|---|
| ClaI | 24 |
| HindIII | 29 |
| NheI | 229 |
| BamHI | 510 |
| XhoI | 515 |
| NdeI | 522 |
| NcoI | 580 |
| XbaI | 619 |
| BglII | 677 |
| SalI | 959 |
| EagI | 1247 |
| NruI | 1282 |
| MscI | 1754 |
| PvuII | 2374 |
| PstI | 3921 |
| AatII | 4598 |
| EcoRI | 4669 |
质粒序列:
| 1 | ttctcatgtt | tgacagctta | tcatcgataa | gctttaatgc | ggtagtttat | 50 |
| 51 | cacagttaaa | ttgctaacgc | agtcaggcac | cgtgtatgaa | atctaacaat | 100 |
| 101 | gcgctcatcg | tcatcctcgg | caccgtcacc | ctggatgctg | taggcatagg | 150 |
| 151 | cttggttatg | ccggtactgc | cgggcctctt | gcgggatatc | gtccattccg | 200 |
| 201 | acagcatcgc | cagtcactat | ggcgtgctgc | tagcgctata | tgcgttgatg | 250 |
| 251 | caatttctat | gcgcacccgt | tctcggagca | ctgtccgacc | gctttggccg | 300 |
| 301 | ccgcccagtc | ctgctcgctt | cgctacttgg | agccactatc | gactacgcga | 350 |
| 351 | tcatggcgac | cacacccgtc | ctgtggatat | ccggatatag | ttcctccttt | 400 |
| 401 | cagcaaaaaa | cccctcaaga | cccgtttaga | ggccccaagg | ggttatgcta | 450 |
| 451 | gttattgctc | agcggtggca | gcagccaact | cagcttcctt | tcgggctttg | 500 |
| 501 | ttagcagccg | gatcctcgag | catatggctg | ccgcgcggca | ccaggccgct | 550 |
| 551 | gctgtgatga | tgatgatgat | ggctgctgcc | catggtatat | ctccttctta | 600 |
| 601 | aagttaaaca | aaattatttc | tagagggaaa | ccgttgtggt | ctccctatag | 650 |
| 651 | tgagtcgtat | taatttcgcg | ggatcgagat | ctcgatcctc | tacgccggac | 700 |
| 701 | gcatcgtggc | cggcatcacc | ggcgccacag | gtgcggttgc | tggcgcctat | 750 |
| 751 | atcgccgaca | tcaccgatgg | ggaagatcgg | gctcgccact | tcgggctcat | 800 |
| 801 | gagcgcttgt | ttcggcgtgg | gtatggtggc | aggccccgtg | gccgggggac | 850 |
| 851 | tgttgggcgc | catctccttg | catgcaccat | tccttgcggc | ggcggtgctc | 900 |
| 901 | aacggcctca | acctactact | gggctgcttc | ctaatgcagg | agtcgcataa | 950 |
| 951 | gggagagcgt | cgaccgatgc | ccttgagagc | cttcaaccca | gtcagctcct | 1000 |
| 1001 | tccggtgggc | gcggggcatg | actatcgtcg | ccgcacttat | gactgtcttc | 1050 |
| 1051 | tttatcatgc | aactcgtagg | acaggtgccg | gcagcgctct | gggtcatttt | 1100 |
| 1101 | cggcgaggac | cgctttcgct | ggagcgcgac | gatgatcggc | ctgtcgcttg | 1150 |
| 1151 | cggtattcgg | aatcttgcac | gccctcgctc | aagccttcgt | cactggtccc | 1200 |
| 1201 | gccaccaaac | gtttcggcga | gaagcaggcc | attatcgccg | gcatggcggc | 1250 |
| 1251 | cgacgcgctg | ggctacgtct | tgctggcgtt | cgcgacgcga | ggctggatgg | 1300 |
| 1301 | ccttccccat | tatgattctt | ctcgcttccg | gcggcatcgg | gatgcccgcg | 1350 |
| 1351 | ttgcaggcca | tgctgtccag | gcaggtagat | gacgaccatc | agggacagct | 1400 |
| 1401 | tcaaggatcg | ctcgcggctc | ttaccagcct | aacttcgatc | actggaccgc | 1450 |
| 1451 | tgatcgtcac | ggcgatttat | gccgcctcgg | cgagcacatg | gaacgggttg | 1500 |
| 1501 | gcatggattg | taggcgccgc | cctatacctt | gtctgcctcc | ccgcgttgcg | 1550 |
| 1551 | tcgcggtgca | tggagccggg | ccacctcgac | ctgaatggaa | gccggcggca | 1600 |
| 1601 | cctcgctaac | ggattcacca | ctccaagaat | tggagccaat | caattcttgc | 1650 |
| 1651 | ggagaactgt | gaatgcgcaa | accaaccctt | ggcagaacat | atccatcgcg | 1700 |
| 1701 | tccgccatct | ccagcagccg | cacgcggcgc | atctcgggca | gcgttgggtc | 1750 |
| 1751 | ctggccacgg | gtgcgcatga | tcgtgctcct | gtcgttgagg | acccggctag | 1800 |
| 1801 | gctggcgggg | ttgccttact | ggttagcaga | atgaatcacc | gatacgcgag | 1850 |
| 1851 | cgaacgtgaa | gcgactgctg | ctgcaaaacg | tctgcgacct | gagcaacaac | 1900 |
| 1901 | atgaatggtc | ttcggtttcc | gtgtttcgta | aagtctggaa | acgcggaagt | 1950 |
| 1951 | cagcgccctg | caccattatg | ttccggatct | gcatcgcagg | atgctgctgg | 2000 |
| 2001 | ctaccctgtg | gaacacctac | atctgtatta | acgaagcgct | ggcattgacc | 2050 |
| 2051 | ctgagtgatt | tttctctggt | cccgccgcat | ccataccgcc | agttgtttac | 2100 |
| 2101 | cctcacaacg | ttccagtaac | cgggcatgtt | catcatcagt | aacccgtatc | 2150 |
| 2151 | gtgagcatcc | tctctcgttt | catcggtatc | attaccccca | tgaacagaaa | 2200 |
| 2201 | tcccccttac | acggaggcat | cagtgaccaa | acaggaaaaa | accgccctta | 2250 |
| 2251 | acatggcccg | ctttatcaga | agccagacat | taacgcttct | ggagaaactc | 2300 |
| 2301 | aacgagctgg | acgcggatga | acaggcagac | atctgtgaat | cgcttcacga | 2350 |
| 2351 | ccacgctgat | gagctttacc | gcagctgcct | cgcgcgtttc | ggtgatgacg | 2400 |
| 2401 | gtgaaaacct | ctgacacatg | cagctcccgg | agacggtcac | agcttgtctg | 2450 |
| 2451 | taagcggatg | ccgggagcag | acaagcccgt | cagggcgcgt | cagcgggtgt | 2500 |
| 2501 | tggcgggtgt | cggggcgcag | ccatgaccca | gtcacgtagc | gatagcggag | 2550 |
| 2551 | tgtatactgg | cttaactatg | cggcatcaga | gcagattgta | ctgagagtgc | 2600 |
| 2601 | accatatatg | cggtgtgaaa | taccgcacag | atgcgtaagg | agaaaatacc | 2650 |
| 2651 | gcatcaggcg | ctcttccgct | tcctcgctca | ctgactcgct | gcgctcggtc | 2700 |
| 2701 | gttcggctgc | ggcgagcggt | atcagctcac | tcaaaggcgg | taatacggtt | 2750 |
| 2751 | atccacagaa | tcaggggata | acgcaggaaa | gaacatgtga | gcaaaaggcc | 2800 |
| 2801 | agcaaaaggc | caggaaccgt | aaaaaggccg | cgttgctggc | gtttttccat | 2850 |
| 2851 | aggctccgcc | cccctgacga | gcatcacaaa | aatcgacgct | caagtcagag | 2900 |
| 2901 | gtggcgaaac | ccgacaggac | tataaagata | ccaggcgttt | ccccctggaa | 2950 |
| 2951 | gctccctcgt | gcgctctcct | gttccgaccc | tgccgcttac | cggatacctg | 3000 |
| 3001 | tccgcctttc | tcccttcggg | aagcgtggcg | ctttctcata | gctcacgctg | 3050 |
| 3051 | taggtatctc | agttcggtgt | aggtcgttcg | ctccaagctg | ggctgtgtgc | 3100 |
| 3101 | acgaaccccc | cgttcagccc | gaccgctgcg | ccttatccgg | taactatcgt | 3150 |
| 3151 | cttgagtcca | acccggtaag | acacgactta | tcgccactgg | cagcagccac | 3200 |
| 3201 | tggtaacagg | attagcagag | cgaggtatgt | aggcggtgct | acagagttct | 3250 |
| 3251 | tgaagtggtg | gcctaactac | ggctacacta | gaaggacagt | atttggtatc | 3300 |
| 3301 | tgcgctctgc | tgaagccagt | taccttcgga | aaaagagttg | gtagctcttg | 3350 |
| 3351 | atccggcaaa | caaaccaccg | ctggtagcgg | tggttttttt | gtttgcaagc | 3400 |
| 3401 | agcagattac | gcgcagaaaa | aaaggatctc | aagaagatcc | tttgatcttt | 3450 |
| 3451 | tctacggggt | ctgacgctca | gtggaacgaa | aactcacgtt | aagggatttt | 3500 |
| 3501 | ggtcatgaga | ttatcaaaaa | ggatcttcac | ctagatcctt | ttaaattaaa | 3550 |
| 3551 | aatgaagttt | taaatcaatc | taaagtatat | atgagtaaac | ttggtctgac | 3600 |
| 3601 | agttaccaat | gcttaatcag | tgaggcacct | atctcagcga | tctgtctatt | 3650 |
| 3651 | tcgttcatcc | atagttgcct | gactccccgt | cgtgtagata | actacgatac | 3700 |
| 3701 | gggagggctt | accatctggc | cccagtgctg | caatgatacc | gcgagaccca | 3750 |
| 3751 | cgctcaccgg | ctccagattt | atcagcaata | aaccagccag | ccggaagggc | 3800 |
| 3801 | cgagcgcaga | agtggtcctg | caactttatc | cgcctccatc | cagtctatta | 3850 |
| 3851 | attgttgccg | ggaagctaga | gtaagtagtt | cgccagttaa | tagtttgcgc | 3900 |
| 3901 | aacgttgttg | ccattgctgc | aggcatcgtg | gtgtcacgct | cgtcgtttgg | 3950 |
| 3951 | tatggcttca | ttcagctccg | gttcccaacg | atcaaggcga | gttacatgat | 4000 |
| 4001 | cccccatgtt | gtgcaaaaaa | gcggttagct | ccttcggtcc | tccgatcgtt | 4050 |
| 4051 | gtcagaagta | agttggccgc | agtgttatca | ctcatggtta | tggcagcact | 4100 |
| 4101 | gcataattct | cttactgtca | tgccatccgt | aagatgcttt | tctgtgactg | 4150 |
| 4151 | gtgagtactc | aaccaagtca | ttctgagaat | agtgtatgcg | gcgaccgagt | 4200 |
| 4201 | tgctcttgcc | cggcgtcaac | acgggataat | accgcgccac | atagcagaac | 4250 |
| 4251 | tttaaaagtg | ctcatcattg | gaaaacgttc | ttcggggcga | aaactctcaa | 4300 |
| 4301 | ggatcttacc | gctgttgaga | tccagttcga | tgtaacccac | tcgtgcaccc | 4350 |
| 4351 | aactgatctt | cagcatcttt | tactttcacc | agcgtttctg | ggtgagcaaa | 4400 |
| 4401 | aacaggaagg | caaaatgccg | caaaaaaggg | aataagggcg | acacggaaat | 4450 |
| 4451 | gttgaatact | catactcttc | ctttttcaat | attattgaag | catttatcag | 4500 |
| 4501 | ggttattgtc | tcatgagcgg | atacatattt | gaatgtattt | agaaaaataa | 4550 |
| 4551 | acaaataggg | gttccgcgca | catttccccg | aaaagtgcca | cctgacgtct | 4600 |
| 4601 | aagaaaccat | tattatcatg | acattaacct | ataaaaatag | gcgtatcacg | 4650 |
| 4651 | aggccctttc | gtcttcaaga | a | 4671 |
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pET-14b
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