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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
pET-43.1b(+)
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号: 60908-2824
质粒名称: pET-43.1b(+)
其他名称:
质粒大小（bp）: 7274
抗性: Ampicillin (氨苄 青 霉 素)
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: 高
蛋白标签: C-HSV, N-His, C-His, N-Thrombin, N-Nus, N-EK
5′ 测序引物及序列: T7: 5′-TAATACGACTCACTATAGGG-3′
3′ 测序引物及序列: ColiDOWN: 5′-TTCACTTCTGAGTTCGGCATG-3′
GenBank号:
其他属性:
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| T7_Terminal_primer | 69 | 87 | |
| rrnB_T2_terminator | 168 | 141 | |
| rrnB_T1_terminator | 343 | 300 | |
| rrnB_terminator | 466 | 309 | |
| EK | 694 | 680 | |
| S15 | 790 | 746 | |
| 6xHIS | 817 | 800 | |
| T7_transl_en_RBS | 2342 | 2326 | |
| lacO | 2387 | 2360 | |
| T7_promoter | 2405 | 2387 | |
| tet (300 – 563) | 2441 | 2704 | |
| pBRrevBam_primer | 2512 | 2493 | |
| lacI | 2787 | 3878 | |
| ROP | 4450 | 4641 | |
| pGEX_3_primer | 4657 | 4635 | |
| pBR322_origin | 5675 | 5056 | |
| AmpR_promoter | 5762 | 5790 | |
| Ampicillin | 5833 | 6693 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 2 | 2317 | 509 | |
| ORF frame 2 | 803 | 2131 | |
| ORF frame 2 | 2318 | 3046 | |
| ORF frame 3 | 2919 | 3878 | |
| ORF frame 1 | 5833 | 6693 |
| Enzyme Name | Cut |
|---|---|
| PacI | 503 |
| XhoI | 530 |
| PmlI | 576 |
| NotI | 592 |
| EagI | 592 |
| HindIII | 599 |
| KpnI | 624 |
| SalI | 626 |
| PstI | 639 |
| AscI | 641 |
| EcoRI | 654 |
| BamHI | 660 |
| SacI | 672 |
| SmaI | 720 |
| XmaI | 718 |
| SacII | 796 |
| SpeI | 827 |
| BglII | 942 |
| MscI | 1074 |
| StuI | 1127 |
| NcoI | 1402 |
| NdeI | 2316 |
| XbaI | 2354 |
| ApaI | 3357 |
| HpaI | 3652 |
| FspI | 6397 |
质粒序列:
| 1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
| 51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggata | catatttgaa | 100 |
| 101 | tgtatttaga | aaaataaaca | aaagagtttg | tagaaacgca | aaaaggccat | 150 |
| 151 | ccgtcaggat | ggccttctgc | ttaatttgat | gcctggcagt | ttatggcggg | 200 |
| 201 | cgtcctgccc | gccaccctcc | gggccgttgc | ttcgcaacgt | tcaaatccgc | 250 |
| 251 | tcccggcgga | tttgtcctac | tcaggagagc | gttcaccgac | aaacaacaga | 300 |
| 301 | taaaacgaaa | ggcccagtct | ttcgactgag | cctttcgttt | tatttgatgc | 350 |
| 351 | ctggcagttc | cctactctcg | catggggaga | ccccacacta | ccatcggcgc | 400 |
| 401 | tacggcgttt | cacttctgag | ttcggcatgg | ggtcaggtgg | gaccaccgcg | 450 |
| 451 | ctactgccgc | caggcaaatt | ctgttttatc | agcctaggaa | cgcccaactt | 500 |
| 501 | aattaacatt | agtggtggtg | gtggtggtgc | tcgagatcct | ctgggtcttc | 550 |
| 551 | aggagcgagt | tctggctggc | ttgcacgtgt | atacagctgt | gcggccgcaa | 600 |
| 601 | gcttcgaacg | cgtatcgatg | gtaccgtcga | cgtcctgcag | gcgcgcctgt | 650 |
| 651 | acagaattcg | gatccacgag | ctccggactc | ttgtcgtcgt | catcaatcgt | 700 |
| 701 | accagaaccc | gcgctgcccc | gggggaccag | accagttggc | ggtggcgagt | 750 |
| 751 | ccatgtgctg | gcgttcaaat | ttcgcagcag | cggtttcttt | acccgcggag | 800 |
| 801 | tgatggtgat | ggtgatgacc | agaaccacta | gtcgcttcgt | caccgaacca | 850 |
| 851 | gcaaatatta | cgggcagcca | taatcagtgc | tccggctttt | tcgtcggtca | 900 |
| 901 | acccttcgat | atcagccaga | tcatcaatgc | cctgttcggc | gagatcttcc | 950 |
| 951 | agcgtacaaa | cgccacgggc | ggccagtttg | aatgccaaat | cacgatctac | 1000 |
| 1001 | cccttcaagg | ttcagcagat | cgtcagccgg | tttgttatca | ccgaggcttt | 1050 |
| 1051 | cttcctgggc | ctgtgcaatg | gtggccagtg | catttttagc | acgctcgcgc | 1100 |
| 1101 | agtgcttcaa | cggtcggctc | atcaaggcct | tcgatttcca | acagctcttt | 1150 |
| 1151 | catcggcaca | taggccaatt | cttccagcgt | cgagaagcct | tcttctacca | 1200 |
| 1201 | gaacagtcgc | gaagtcttcg | tcgatgtcga | gatatttggt | gaaggtgtcg | 1250 |
| 1251 | atcgctgcgt | gcgcttccgc | ctgatgctta | gcttgcaggt | cgtcaacggt | 1300 |
| 1301 | catcacgttg | agttcccaac | cgctcagttg | cgaagccaga | cgcacgttct | 1350 |
| 1351 | gaccgttacg | gccaatcgcc | tgcgccagat | taccggcttc | aacggcgatg | 1400 |
| 1401 | tccatggtgt | gtttatcttc | atccaccacg | atagaagcaa | cgtctgccgg | 1450 |
| 1451 | tgccattgcg | ttaatcacga | actgcgccgg | gttatcatcc | cacaggacga | 1500 |
| 1501 | tatcgatacg | ctcgccaccc | agttcagtag | acaccgcctg | aacacgcgcg | 1550 |
| 1551 | ccacgcatac | ctacgcaagc | acctaccgga | tcgatacgtt | tatcgttggt | 1600 |
| 1601 | tttcaccgcg | attttcgcac | gagaacccgg | atcgcgagcc | gctgctttaa | 1650 |
| 1651 | tttcaatcac | ttcttcgccg | atttctggca | cttcaatacg | gaacagttcg | 1700 |
| 1701 | atcagcattt | ccggcttgga | acgagtgacg | aacagttgcg | cgccacgcgc | 1750 |
| 1751 | ttccgggcga | acggaataga | gcacgccacg | aacgcggtcg | ccagggcgga | 1800 |
| 1801 | agttttcacg | cggcagcata | tcttcgcgca | ggatcacggc | ttcagcgttg | 1850 |
| 1851 | ttgcccagat | ccagagagat | gttgtcgcgg | tttacttttt | tcaccacgcc | 1900 |
| 1901 | ggtgatgatt | tcaccttcgt | gttcacggaa | ctgatcaacc | accatcgcac | 1950 |
| 1951 | gttcggcttc | acgcactttc | tgcacgataa | cctgttttgc | cgtctgggta | 2000 |
| 2001 | gtgatacggt | caaaggtaac | agactcaatc | tgatcttcaa | cgtaatcgcc | 2050 |
| 2051 | caggttcagg | ctttcatctt | cataacgtgc | ggcttcaagg | gtgatttcct | 2100 |
| 2101 | tggtcggctg | ggtgacttca | tcaacaacta | accagcgacg | gaaagtgtca | 2150 |
| 2151 | aaatcaccgc | ttttgcgatc | gatctgtacg | cggacgtcga | tctcttgttc | 2200 |
| 2201 | atattttttc | tttgttgctg | tcgccagcgc | gctttccaat | gcttcgaaaa | 2250 |
| 2251 | tcttctcgcg | aggtagcgcc | ttttcattgg | atacggcttc | aactacagcc | 2300 |
| 2301 | aaaatttctt | tgttcatatg | tatatctcct | tcttaaagtt | aaacaaaatt | 2350 |
| 2351 | atttctagag | gggaattgtt | atccgctcac | aattccccta | tagtgagtcg | 2400 |
| 2401 | tattaatttc | gcgggatcga | gatcgatctc | gatcctctac | gccggacgca | 2450 |
| 2451 | tcgtggccgg | catcaccggc | gccacaggtg | cggttgctgg | cgcctatatc | 2500 |
| 2501 | gccgacatca | ccgatgggga | agatcgggct | cgccacttcg | ggctcatgag | 2550 |
| 2551 | cgcttgtttc | ggcgtgggta | tggtggcagg | ccccgtggcc | gggggactgt | 2600 |
| 2601 | tgggcgccat | ctccttgcat | gcaccattcc | ttgcggcggc | ggtgctcaac | 2650 |
| 2651 | ggcctcaacc | tactactggg | ctgcttccta | atgcaggagt | cgcataaggg | 2700 |
| 2701 | agagcgtcga | gatcccggac | accatcgaat | ggcgcaaaac | ctttcgcggt | 2750 |
| 2751 | atggcatgat | agcgcccgga | agagagtcaa | ttcagggtgg | tgaatgtgaa | 2800 |
| 2801 | accagtaacg | ttatacgatg | tcgcagagta | tgccggtgtc | tcttatcaga | 2850 |
| 2851 | ccgtttcccg | cgtggtgaac | caggccagcc | acgtttctgc | gaaaacgcgg | 2900 |
| 2901 | gaaaaagtgg | aagcggcgat | ggcggagctg | aattacattc | ccaaccgcgt | 2950 |
| 2951 | ggcacaacaa | ctggcgggca | aacagtcgtt | gctgattggc | gttgccacct | 3000 |
| 3001 | ccagtctggc | cctgcacgcg | ccgtcgcaaa | ttgtcgcggc | gattaaatct | 3050 |
| 3051 | cgcgccgatc | aactgggtgc | cagcgtggtg | gtgtcgatgg | tagaacgaag | 3100 |
| 3101 | cggcgtcgaa | gcctgtaaag | cggcggtgca | caatcttctc | gcgcaacgcg | 3150 |
| 3151 | tcagtgggct | gatcattaac | tatccgctgg | atgaccagga | tgccattgct | 3200 |
| 3201 | gtggaagctg | cctgcactaa | tgttccggcg | ttatttcttg | atgtctctga | 3250 |
| 3251 | ccagacaccc | atcaacagta | ttattttctc | ccatgaagac | ggtacgcgac | 3300 |
| 3301 | tgggcgtgga | gcatctggtc | gcattgggtc | accagcaaat | cgcgctgtta | 3350 |
| 3351 | gcgggcccat | taagttctgt | ctcggcgcgt | ctgcgtctgg | ctggctggca | 3400 |
| 3401 | taaatatctc | actcgcaatc | aaattcagcc | gatagcggaa | cgggaaggcg | 3450 |
| 3451 | actggagtgc | catgtccggt | tttcaacaaa | ccatgcaaat | gctgaatgag | 3500 |
| 3501 | ggcatcgttc | ccactgcgat | gctggttgcc | aacgatcaga | tggcgctggg | 3550 |
| 3551 | cgcaatgcgc | gccattaccg | agtccgggct | gcgcgttggt | gcggacatct | 3600 |
| 3601 | cggtagtggg | atacgacgat | accgaagaca | gctcatgtta | tatcccgccg | 3650 |
| 3651 | ttaaccacca | tcaaacagga | ttttcgcctg | ctggggcaaa | ccagcgtgga | 3700 |
| 3701 | ccgcttgctg | caactctctc | agggccaggc | ggtgaagggc | aatcagctgt | 3750 |
| 3751 | tgcccgtctc | actggtgaaa | agaaaaacca | ccctggcgcc | caatacgcaa | 3800 |
| 3801 | accgcctctc | cccgcgcgtt | ggccgattca | ttaatgcagc | tggcacgaca | 3850 |
| 3851 | ggtttcccga | ctggaaagcg | ggcagtgagc | gcaacgcaat | taatgtaagt | 3900 |
| 3901 | tagctcactc | attaggcacc | gggatctcga | ccgatgccct | tgagagcctt | 3950 |
| 3951 | caacccagtc | agctccttcc | ggtgggcgcg | gggcatgact | agcatgatcg | 4000 |
| 4001 | tgctcctgtc | gttgaggacc | cggctaggct | ggcggggttg | ccttactggt | 4050 |
| 4051 | tagcagaatg | aatcaccgat | acgcgagcga | acgtgaagcg | actgctgctg | 4100 |
| 4101 | caaaacgtct | gcgacctgag | caacaacatg | aatggtcttc | ggtttccgtg | 4150 |
| 4151 | tttcgtaaag | tctggaaacg | cggaagtcag | cgccctgcac | cattatgttc | 4200 |
| 4201 | cggatctgca | tcgcaggatg | ctgctggcta | ccctgtggaa | cacctacatc | 4250 |
| 4251 | tgtattaacg | aagcgctggc | attgaccctg | agtgattttt | ctctggtccc | 4300 |
| 4301 | gccgcatcca | taccgccagt | tgtttaccct | cacaacgttc | cagtaaccgg | 4350 |
| 4351 | gcatgttcat | catcagtaac | ccgtatcgtg | agcatcctct | ctcgtttcat | 4400 |
| 4401 | cggtatcatt | acccccatga | acagaaatcc | cccttacacg | gaggcatcag | 4450 |
| 4451 | tgaccaaaca | ggaaaaaacc | gcccttaaca | tggcccgctt | tatcagaagc | 4500 |
| 4501 | cagacattaa | cgcttctgga | gaaactcaac | gagctggacg | cggatgaaca | 4550 |
| 4551 | ggcagacatc | tgtgaatcgc | ttcacgacca | cgctgatgag | ctttaccgca | 4600 |
| 4601 | gctgcctcgc | gcgtttcggt | gatgacggtg | aaaacctctg | acacatgcag | 4650 |
| 4651 | ctcccggaga | cggtcacagc | ttgtctgtaa | gcggatgccg | ggagcagaca | 4700 |
| 4701 | agcccgtcag | ggcgcgtcag | cgggtgttgg | cgggtgtcgg | ggcgcagcca | 4750 |
| 4751 | tgacccagtc | acgtagcgat | agcggagtgt | atactggctt | aactatgcgg | 4800 |
| 4801 | catcagagca | gattgtactg | agagtgcacc | atatatgcgg | tgtgaaatac | 4850 |
| 4851 | cgcacagatg | cgtaaggaga | aaataccgca | tcaggcgctc | ttccgcttcc | 4900 |
| 4901 | tcgctcactg | actcgctgcg | ctcggtcgtt | cggctgcggc | gagcggtatc | 4950 |
| 4951 | agctcactca | aaggcggtaa | tacggttatc | cacagaatca | ggggataacg | 5000 |
| 5001 | caggaaagaa | catgtgagca | aaaggccagc | aaaaggccag | gaaccgtaaa | 5050 |
| 5051 | aaggccgcgt | tgctggcgtt | tttccatagg | ctccgccccc | ctgacgagca | 5100 |
| 5101 | tcacaaaaat | cgacgctcaa | gtcagaggtg | gcgaaacccg | acaggactat | 5150 |
| 5151 | aaagatacca | ggcgtttccc | cctggaagct | ccctcgtgcg | ctctcctgtt | 5200 |
| 5201 | ccgaccctgc | cgcttaccgg | atacctgtcc | gcctttctcc | cttcgggaag | 5250 |
| 5251 | cgtggcgctt | tctcatagct | cacgctgtag | gtatctcagt | tcggtgtagg | 5300 |
| 5301 | tcgttcgctc | caagctgggc | tgtgtgcacg | aaccccccgt | tcagcccgac | 5350 |
| 5351 | cgctgcgcct | tatccggtaa | ctatcgtctt | gagtccaacc | cggtaagaca | 5400 |
| 5401 | cgacttatcg | ccactggcag | cagccactgg | taacaggatt | agcagagcga | 5450 |
| 5451 | ggtatgtagg | cggtgctaca | gagttcttga | agtggtggcc | taactacggc | 5500 |
| 5501 | tacactagaa | ggacagtatt | tggtatctgc | gctctgctga | agccagttac | 5550 |
| 5551 | cttcggaaaa | agagttggta | gctcttgatc | cggcaaacaa | accaccgctg | 5600 |
| 5601 | gtagcggtgg | tttttttgtt | tgcaagcagc | agattacgcg | cagaaaaaaa | 5650 |
| 5651 | ggatctcaag | aagatccttt | gatcttttct | acggggtctg | acgctcagtg | 5700 |
| 5701 | gaacgaaaac | tcacgttaag | ggattttggt | catgacctat | ttgtttattt | 5750 |
| 5751 | ttctaaatac | attcaaatat | gtatccgctc | atgagacaat | aaccctgata | 5800 |
| 5801 | aatgcttcaa | tcatgattga | aaaaggaaga | gtatgagtat | tcaacatttc | 5850 |
| 5851 | cgtgtcgccc | ttattccctt | ttttgcggca | ttttgccttc | ctgtttttgc | 5900 |
| 5901 | tcacccagaa | acgctggtga | aagtaaaaga | tgctgaagat | cagttgggtg | 5950 |
| 5951 | cacgagtggg | ttacatcgaa | ctggatctca | acagcggtaa | gatccttgag | 6000 |
| 6001 | agttttcgcc | ccgaagaacg | ttttccaatg | atgagcactt | ttaaagttct | 6050 |
| 6051 | gctatgtggc | gcggtattat | cccgtattga | cgccgggcaa | gagcaactcg | 6100 |
| 6101 | gtcgccgcat | acactattct | cagaatgact | tggttgagta | ctcaccagtc | 6150 |
| 6151 | acagaaaagc | atcttacgga | tggcatgaca | gtaagagaat | tatgcagtgc | 6200 |
| 6201 | tgccataacc | atgagtgata | acactgcggc | caacttactt | ctgacaacga | 6250 |
| 6251 | tcggaggacc | gaaggagcta | accgcttttt | tgcacaacat | gggggatcat | 6300 |
| 6301 | gtaactcgcc | ttgatcgttg | ggaaccggag | ctgaatgaag | ccataccaaa | 6350 |
| 6351 | cgacgagcgt | gacaccacga | tgcctgtagc | aatggcaaca | acgttgcgca | 6400 |
| 6401 | aactattaac | tggcgaacta | cttactctag | cttcccggca | acaattaata | 6450 |
| 6451 | gactggatgg | aggcggataa | agttgcagga | ccacttctgc | gctcggccct | 6500 |
| 6501 | tccggctggc | tggtttattg | ctgataaatc | tggagccggt | gagcgtgggt | 6550 |
| 6551 | ctcgcggtat | cattgcagca | ctggggccag | atggtaagcc | ctcccgtatc | 6600 |
| 6601 | gtagttatct | acacgacggg | gagtcaggca | actatggatg | aacgaaatag | 6650 |
| 6651 | acagatcgct | gagataggtg | cctcactgat | taagcattgg | taactgtcag | 6700 |
| 6701 | accaagttta | ctcatatata | ctttagattg | atttaaaact | tcatttttaa | 6750 |
| 6751 | tttaaaagga | tctaggtgaa | gatccttttt | gataatctca | tgccatcgtg | 6800 |
| 6801 | ccgccagaaa | ttgtaaacgt | taatattttg | ttaaaattcg | cgttaaattt | 6850 |
| 6851 | ttgttaaatc | agctcatttt | ttaaccaata | ggccgaaatc | ggcaaaatcc | 6900 |
| 6901 | cttataaatc | aaaagaatag | accgagatag | ggttgagtgt | tgttccagtt | 6950 |
| 6951 | tggaacaaga | gtccactatt | aaagaacgtg | gactccaacg | tcaaagggcg | 7000 |
| 7001 | aaaaaccgtc | tatcagggcg | atggcccact | acgtgaacca | tcaccctaat | 7050 |
| 7051 | caagtttttt | ggggtcgagg | tgccgtaaag | cactaaatcg | gaaccctaaa | 7100 |
| 7101 | gggagccccc | gatttagagc | ttgacgggga | aagccggcga | acgtggcgag | 7150 |
| 7151 | aaaggaaggg | aagaaagcga | aaggagcggg | cgctagggcg | ctggcaagtg | 7200 |
| 7201 | tagcggtcac | gctgcgcgta | accaccacac | ccgccgcgct | taatgcgccg | 7250 |
| 7251 | ctacagggcg | cgtcccattc | gcca | 7274 |
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pET-43.1b(+)
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