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- 详细信息
- 文献和实验
- 技术资料
- 库存:
1000
- 英文名:
Recombinant Calcitonin Receptor (CTR)
- 保质期:
12个月
- 供应商:
上海再康
- 保存条件:
-20℃
| Organism species | Rattus norvegicus (Rat) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 17.1kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Val34~Tyr148 with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
VSPTPVL QAPTNLTDSG LDQEPFLYLV GRKKLLDAQY KCYDRIQQLP PYEGEGPYCN RTWDGWMCWD DTPAGVMSYQ HCPDYFPDFD PTEKVSKYCD ENGEWFRHPD SNRTWSNY
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验Press 3、 Medicinal Protein Engineering Published: December 01, 2008 by CRC Press - 584 Pages Editor(s): Yury E. Khudyakov, Division of Viral Hepatitis, Nat'l Ctr. Infect. Dis., CDCP 4、 Protein Engineering Pub. Date: November
吴佰霖 老板的一个课题,一个分泌性蛋白,可结合一些小分子,与膜上受体结合,但是用重组表达的蛋白与 pcDNA过表达这个蛋白的生物学效果不一样 请问有什么研究策略吗 freecell 你的研究材料? 研究目的? 吴佰霖 材料:细胞系,酵母表达重组蛋白,pcDNA表达克隆载体, 研究目的是分泌性蛋白结合受体后在胞内的信号通路,或相关作用分子
,建议选择 OD 值落在标曲范围中部的稀释倍数为最佳稀释倍数。 2 回收率 评估试剂盒测量结果趋于真实值程度的指标。一般添加标准品(重组蛋白)到天然样本基质内,进行测定。通常,线性及回收率的范围在 80%-120% 比较合适。 3 天然样本检测 确保试剂盒可以检测天然样本及合适的样本类型。可根据靶蛋白性质对天然样本进行选择,例如:血清、血浆、细胞上清、尿液、唾液和乳汁等。 4 交叉反应 通过交叉反应评估试剂盒的特异性。交叉反应越小,说明试剂盒的特异性越好。 ✔与同家族其他蛋白或结构类似物的交叉
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