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文献和实验cells. We present here (i) a detailed protocol for the production and purification of soluble recombinant NK cell receptors tagged with human IgG1-Fc (thus termed receptor-Fc chimera or receptor-Ig fusion protein) and (ii) a protocol for cell staining
Exploiting Fc Chimaeric Proteins for the Identification of Ligands Specific for the Mannose Receptor
. In this chapter we describe our experience in the use of proteins containing selected regions of the mannose receptor fused to the Fc region of human IgG1 or murine IgG2b. Using these reagents we have discovered new and unexpected ligands for the mannose receptor
Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
the isolation of activated Fc receptor complexes from RAW 264.7 macrophages using live?cell affinity receptor chromatography (LARC). The Fc receptor complex is activated and captured by IgG?coated microbeads on the surface of live macrophages. After the cells
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