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文献和实验The Maillard Reaction Induced Modifications of Endogenous Opioid Peptide Enkephalin
Nonenzymatic glycation (Maillard reaction) is a posttranslational modification of peptides and proteins by sugars, which, after a cascade of reactions, leads to the formation of a complex family of irreversibly changed advanced glycation end
Peptide Library Construction from RNA-PCR-Derived RNAs
The generation of peptide from messenger RNA (mRNA) provides a convenient source for current proteomic analysis. Intron-free mRNA possessing adenine-uracil-guanine (AUG) start codons can be translated into labeled or unlabeled peptides
Study of Substrate Specificity of MAPKs Using Oriented Peptide Libraries
, particularly mass spectroscopy, has greatly facilitated this analysis (1 –3 ), although usually the investigator must still perform large scale biochemical purification of the presumed substrate to determine its identity. The use of oriented peptide library
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