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- 详细信息
- 文献和实验
- 技术资料
- 克隆性:
Polyclonal
- 抗体名:
Cy7标记的磷酸化RNA聚合酶II CTD抗体
- 宿主:
Rabbit
- 形态:
Lyophilized or Liquid
- 应用范围:
ICC=1:50-200 IF=1:50-200
- 浓度:
1mg/ml
- 保存条件:
-20°C
- 规格:
100ul
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文献和实验mRNA Amplification with T7 RNA Polymerase
Materials MessageAmp II aRNA Amplication Kit (Cat #1751, Ambion) 100% Ethanol RNA samples (e.g. 1 µg RNA per sample) Procedure Reverse transcription Verify that EtOH (24 mL) has been added to the Wash Buffer. Turn
Cloning of Taq polymerase-amplified PCR products
实验原理 The plasmid vector (pCR®II-TOPO® or pCR®2.1-TOPO®) is supplied linearized with: Single 3´-thymidine (T) overhangs for TA Cloning® Topoisomerase I covalently bound to the vector (referred to as "activated
96-well RNA In Situ Hybridization Protocol
: 4% paraformaldehyde/PBS) for 5 min.11. Fix 10 min. in 4% paraformaldehyde/PBS.12. Rinse 3X with PBS + 0.1% Tween 20 (PBT).13. Wash embryos 3X with PBT before hybridization.II. PREPARATION OF PROBEThe composition of the buffers used in sections II and III and general
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