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文献和实验Differentiation of Mouse Embryonic Stem Cells in Self-Assembling Peptide Scaffolds
Here, we describe the capacity of mouse embryonic stem cells (mESCs) to differentiate into osteoblast-like cells in a three-dimensional (3D) self-assembling peptide scaffold, a synthetic nanofiber biomaterial with future applications
Peptide Inhibition ELISA Protocol
Dilute specific and non-specific peptides to 200 mg/ml in PBT. In a microtiter plate, mix equal volumes of the peptide dilutions and the MAb supe. Add an equal volume of PBT and MAb as the control. Incubate 1 hour at 37
Assessment of Insulin Secretion in the Mouse
. 1 ). The mouse synthesizes two molecular forms of insulin and C-peptide, which are identical to their respective rat counterparts ( 5 ). The two rodent insulins, designated insulin I and II, are present at a ratio of 1∶3 in the mouse and 4∶1 in the rat
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