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- 详细信息
- 文献和实验
- 技术资料
- 靶点:
见 说 明 书
- 克隆性:
多克隆
- 抗体英文名:
Polyclonal Antibody to S100 Calcium Binding Protein A4 (S100A4)
- 抗体名:
S100钙结合蛋白A4(S100A4)多克隆抗体
- 标记物:
未标记
- 宿主:
Rabbit,兔
- 适应物种:
见 说 明 书
- 免疫原:
见 说 明 书
- 亚型:
见 说 明 书
- 形态:
液态/粉末
- 应用范围:
WB, ICC, IHC-P, IHC-F, ELISA
- 浓度:
200ug/ml
- 保存条件:
-20oC
| Organism species | Bos taurus; Bovine (Cattle) |
| Product No. | ZKC020Bo01 |
| Clonality | Polyclonal |
| Host | Rabbit |
| Immunoglobulin Type | IgG |
| Purification | Affinity Chromatography |
| Applications | WB, ICC, IHC-P, IHC-F, ELISA |
| Concentration | 200ug/ml |
| UOM | 100ug |
| Conjugate | No Conjugate |
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Optimal working dilutions must be determined by end user.
containing 0.02% NaN3, 50% glycerol.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
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文献和实验Characterization of Epithelial Cells in the Hair Follicle With S100 Proteins
proteins are expressed in very restricted regions of the hair follicle: S100A3 and S100A6 in distinct postmitotic differentiated epithelial cells and S100A4 and S100A6 in the epithelial stem cell compartments. Characterization of epithelial cells
胆酸钠(离子去污剂,提取蛋白;用H2O配制成10%储存液;避光保存) 注意:准备激酶(致活酶)实验时,不要加去氧胆酸钠,因为离子型去污剂能够使酶变性,导致活性丧失。 RIPA蛋白酶抑制剂 苯甲基磺酰氟(PMSF)(用异丙醇配制成200mM的储存液,室温保存) EDTA(钙螯合剂;用H2O配制成100mM的储存液,PH 7.4) 亮抑酶肽(Leupeptin)(用H2O配制成1mg/ml的储存液,分装,-20℃保存) 抑蛋白酶肽(Aprotinin)(用H2O配制成1mg/ml的储存
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