- 询价
- R&D
- ZK-0002944
- 美国/中国
- 2025年11月20日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 技术资料
- 靶点：
来电咨询
- 克隆性：
多克隆
- 标记物：
见 说 明 书
- 宿主：
Goat，Rabbit，Mouse
- 适应物种：
人，大鼠，小鼠，兔
- 形态：
液态/粉状
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 保存条件：
-20℃保存
- ACUITY Biotin Free Polymer Detection HRP System 50 Test AEC (SIGNET)详细信息：
-
Description ACUITY Biotin Free Polymer Detection HRP System, AEC, 50 Tests
(Formerly Signet Catalog No.: 2600)Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Form ACUITY Biotin Free Polymer Detection HRP System 50 Test AEC (SIGNET)Detection Kit Specificity Signets ACUITY Detection system is a sensitive biotin-free polymer detection kit that is compatible with antibodies raised in mouse, rat and rabbit. ACUITY contains a horseradish Peroxidase labeled polymer and special blocking solutions to eliminate possible nonspecific background staining.
Kit Contains:- Serum Block, 5 mL
- Special Block, 5 mL
- HRP Polymer Link, 5 mL
- AEC Substrate Buffer, 24 mL
- AEC Chromogen, 1 mL
- Hydrogen Peroxide, 5 mL
Suggested Working Dilution
ACUITY Biotin Free Polymer Detection HRP System 50 Test AEC (SIGNET) Protocol:
1. Tissue preparation: Deparaffinize and rehydrate according to the established protocols.
2. Blocking step-1: Incubate with H202 at RT for 15 minutes. Rinse with PBS.
3. Blocking step-2: Apply the Serum Block reagent for 15 minutes
4. Primary antibody: Apply the primary antibody for 30 minutes
5. Blocking step-3: Apply the Special Block reagent for 20 minutes then rinse with PBS (5min/2X).
6. Polymer Link: Apply the Polymer reagent for 30 minutes then rinse with PBS (5min/2X).
7. Chromogen step: Apply AEC Working Solution for 3-5 minutes then rinse with DI water (see AEC preparation guidelines below).
8. Counter stain & mount.
- AEC Working Solution* Preparation
Buffer + Chromogen = Working Solution
5 mL + 0.10 mL = 5mL
10 mL + 0.20 mL = 10mL
24 mL + 0.48 mL = 24mL
Notes *Discard unused AEC working solution at the end of the run. Storage Store between 2-8°C. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - ACUITY Biotin Free Polymer Detection HRP System 50 Test AEC (SIGNET)
- zkA090Hu 巨噬细胞集落刺激因子(MCSF)ELISA试剂盒 检测范围
zkA091Hu 巨噬细胞来源趋化因子(MDC)ELISA试剂盒 检测范围
zkA102Hu 基质金属蛋白酶7(MMP7)ELISA试剂盒 检测范围
zkA103Hu 基质金属蛋白酶8(MMP8)ELISA试剂盒 检测范围
zkA105Hu 神经生长因子(NGF)ELISA试剂盒 检测范围
zkA106Hu 神经营养因子3(NT3)ELISA试剂盒 检测范围
zkA107Hu 神经营养因子4(NT4)ELISA试剂盒 检测范围
zkA108Hu 骨保护素(OPG)ELISA试剂盒 检测范围
zkA110Hu 抑瘤素M(OSM)ELISA试剂盒 检测范围
zkA114Hu 胎盘生长因子(PLGF)ELISA试剂盒 检测范围
zkA116Hu 调节激活正常T-细胞表达分泌因子(RANTES)ELISA试剂盒 检测范围
zkA118Hu CD30配体(CD30L)ELISA试剂盒 检测范围
zkA119Hu CD40配体(CD40L)ELISA试剂盒 检测范围
zkA120Hu 干细胞因子(SCF)ELISA试剂盒 检测范围
zkA121Hu 干细胞因子受体(SCFR)ELISA试剂盒 检测范围
zkA122Hu 基质细胞衍生因子1(SDF1)ELISA试剂盒 检测范围
zkA123Hu 转化生长因子α(TGFα)ELISA试剂盒 检测范围
zkA124Hu 转化生长因子β1(TGFβ1)ELISA试剂盒 检测范围
zkA125Hu 免疫球蛋白样EGF样域酪氨酸激酶1(Tie1)ELISA试剂盒 检测范围
zkA126Hu 内皮细胞TEK酪氨酸激酶(Tie2)ELISA试剂盒 检测范围
zkA128Hu 组织金属蛋白酶抑制因子2(TIMP2)ELISA试剂盒 检测范围
zkA129Hu 组织金属蛋白酶抑制因子3(TIMP3)ELISA试剂盒 检测范围
zkA130Hu 组织金属蛋白酶抑制因子4(TIMP4)ELISA试剂盒 检测范围
zkA133Hu 肿瘤坏死因子α(TNFα)ELISA试剂盒 检测范围
zkA134Hu 肿瘤坏死因子β(TNFβ)ELISA试剂盒 检测范围
zkA135Hu 血小板生成素(TPO)ELISA试剂盒 检测范围
zkA140Hu 尿激酶型纤溶酶原激活因子(uPA)ELISA试剂盒 检测范围
zkA141Hu 尿激酶型纤溶酶原激活因子受体(uPAR)ELISA试剂盒 检测范围
zkA142Hu 细胞间粘附分子5(ICAM5)ELISA试剂盒 检测范围
zkB851Hu 血管内皮生长因子121(VEGF121)ELISA试剂盒 检测范围
zkA144Hu 血管内皮生长因子B(VEGFB)ELISA试剂盒 检测范围
zkA145Hu 血管内皮生长因子C(VEGFC)ELISA试剂盒 检测范围
zkA146Hu 血管内皮生长因子D(VEGFD)ELISA试剂盒 检测范围
zkA150Hu 癌胚抗原(HZA)ELISA试剂盒 检测范围
zkA151Hu 激肽释放酶3(KLK3)ELISA试剂盒 检测范围
zkA154Hu 糖抗原125(CA125)ELISA试剂盒 检测范围
zkA155Hu 糖抗原15-3(CA15-3)ELISA试剂盒 检测范围
zkA156Hu 糖抗原19-9(CA19-9)ELISA试剂盒 检测范围
zkA159Hu 鳞状细胞癌抗原2(SCCA2)ELISA试剂盒 检测范围
zkA163Hu 组织多肽抗原(TPA)ELISA试剂盒 检测范围
zkA165Hu 胃蛋白酶原A(PGA)ELISA试剂盒 检测范围
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ACUITY Biotin Free Polymer Detection HRP System 50 Test AEC (SIGNET)
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