- 询价
- R&D
- ZK-0002626
- 美国/中国
- 2025年11月20日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 技术资料
- 靶点：
来电咨询
- 克隆性：
多克隆
- 标记物：
见 说 明 书
- 宿主：
Goat，Rabbit，Mouse
- 适应物种：
人，大鼠，小鼠，兔
- 形态：
液态/粉状
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 保存条件：
-20℃保存
- ACTIVITY-SELECT Rabbit PAP保存于运输说明：
- Store at -20°C.
- 详细信息：
-
Description Peroxidase-antiperoxidase complex containing horseradish peroxidase and immunospecifically pure rabbit antiperoxidase. Form Purified Antibody (in PBS, 0.01M thimerosal) Host Rabbit [Ab] ACTIVITY-SELECT Rabbit PAP 1 mg/mL Specificity ACTIVITY-SELECT rabbit PAP (peroxidase-antiperoxidase complex containing horseradish peroxidase and immunospecifically pure rabbit antiperoxidase). Recent modifications in our laboratory of the original PAP isolation procedure have led to PAP preparations of higher activity than those available before.
Rabbit PAP is intended for the detection of first antibody of rabbit origin via an excess of an antirabbit IgG (such as goat antirabbit IgG Cat # SMI-5030c). Rabbit antibodies of any isotype are detectable via reaction with shared light chains.
Composition: Immune complex of horseradish peroxidase and rabbit antiperoxidase providing maximal immunocytochemical staining intensity at a dilution range of 1:100 to 1:1,600. Staining intensity decreases at dilutions of 1:3,200 and beyond.Uses This antibody is primarily used for immunohistochemistry applications, but it is also effective in blotting and ELISA assays. It can be used with diaminobenzidine tetrahydrochloride (DAB) substrates for detection with peroxidase. Suggested Working Dilution ACTIVITY-SELECT Rabbit PAP Because of the higher activity of our newly developed PAP, staining intensity is increased and more extensive dilutions are required with consequently greater economy. Dilution for use in immunocytochemistry depends on tissue preparation, abundance of antigen and quality of the first antibody. Therefore, we recommend that initially PAP be applied in a series of dilutions ranging from 1:100 to 1:1,600. Similar dilutions are recommended for Western blots and ELISA. Concentrations higher than those recommended for PAP or other antibody-based reagents may produce a brown background and decrease specific staining intensity. Notes This antibody is sold for research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. Storage Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - ACTIVITY-SELECT Rabbit PAP
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ACTIVITY-SELECT Rabbit PAP
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