- 询价
- R&D
- ZK-0002422
- 美国/中国
- 2025年11月20日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 靶点：
来电咨询
- 克隆性：
多克隆
- 标记物：
见 说 明 书
- 宿主：
Goat，Rabbit，Mouse
- 适应物种：
人，大鼠，小鼠，兔
- 形态：
液态/粉状
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 保存条件：
-20℃保存
- Placental Alkaline Phosphatase (PLAP) Polyclonal Antibody, Purified (SIGNET)详细信息：
-
Description Purified Polyclonal Antibody against Placental Alkaline Phosphatase (PLAP)
(Formerly Signet Catalog Nos: 258-01, 258-16 L1 Predilute, 258-26 L2 Predilute)Intended Use ** In Vitro Diagnostic (IVD) ** Form Purified Antibody (in 0.01M PBS + 0.1% NaN3 + 1% BSA) Host Rabbit Species Reactivity Placental Alkaline Phosphatase (PLAP) Polyclonal Antibody, Purified (SIGNET)Human Specificity The SIGNET polyclonal Placental Alkaline Phosphatase (PLAP) antibody recognizes trophoblast cells in human placenta. This antibody also detects human intestinal alkaline phosphatase.
This antibody was generated using purified human placental alkaline phosphatase.
Concentrated Format:
SIG-3258-1000 [1 mL]
Prediluted Formats:
SIG-3258-16 [6 mL]
SIG-3258-26 [6 mL] is ready-to-use with Biotin-based detection systems such as Signets USA Ultrastreptavidin Detection (SIG-32250).Uses Placental Alkaline Phosphatase (PLAP) Polyclonal Antibody, Purified (SIGNET)This antibody is effective in immunohistochemistry (IHC). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as Signets USA Ultrastreptavidin Detection (SIG-32250).
Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: Not required
Incubation: 20-60 minutes at room temperature
Notes Positive tissue (human): Germ cell tumor or placenta Storage Store between 2-8°C. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as Signets USA Ultrastreptavidin Detection (SIG-32250).
- Placental Alkaline Phosphatase (PLAP) Polyclonal Antibody, Purified (SIGNET)
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
phosphatase gene, PLAP (Kam et al 1985), is perhaps the most useful as it is very heat stable and is resistant to some chemical inhibitors that are active on other endogenous alkaline phosphatases. PLAP has been used as a histochemical reporter fairly recently
ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
antibody. 30 minutes. 5. Jet wash in tris/HC1 saline buffer pH 7.6. 6. Wash in buffer. 3 minutes. Steps 7-9 for POLYCLONAL Primary Antisera Only 7. Incubate in mouse anti-rabbit immunoglobulins. 30 minutes. 8. Jet wash in buffer.
Staining protocols for placental explant cultures
). Note that alkaline phosphatase conjugates should be used with caution as this enzyme is expressed by some trophoblast. Resin embed allowfullscreen='true'ding of explant cutures. Examination of semithin resin sections can show the pattern of cell
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