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- 文献和实验
- 技术资料
- 库存：
1000
- 英文名：
Recombinant Glutaryl Coenzyme A Dehydrogenase (GCDH)
- 保质期：
12个月
- 供应商：
上海再康
- 保存条件：
-20℃
戊二酰辅酶A脱氢酶(GCDH)重组蛋白
Recombinant Glutaryl Coenzyme A Dehydrogenase (GCDH)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | zk-D681Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 22.1kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Glu270~Lys438 with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
戊二酰辅酶A脱氢酶(GCDH)重组蛋白 SEQUENCES
The sequence of the target protein is listed below.
E VPEENVLPGA SSLGGPFGCL NNARYGIAWG VLGASEFCLH TARQYALDRM QFGVPLARNQ LIQKKLADML TEITLGLHAC LQLGRLKDQD KAAPEMVSLL KRNNCGKALD IARQARDMLG GNGISDEYHV IRHAMNLEAV NTYEGTHDIH ALILGRAITG IQAFTASK
E VPEENVLPGA SSLGGPFGCL NNARYGIAWG VLGASEFCLH TARQYALDRM QFGVPLARNQ LIQKKLADML TEITLGLHAC LQLGRLKDQD KAAPEMVSLL KRNNCGKALD IARQARDMLG GNGISDEYHV IRHAMNLEAV NTYEGTHDIH ALILGRAITG IQAFTASK
USAGE
Reconstitute in sterile ddH2O.
戊二酰辅酶A脱氢酶(GCDH)重组蛋白 STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
戊二酰辅酶A脱氢酶(GCDH)重组蛋白 About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
为巴豆酰辅酶A（S-but-2-enoylcoenzyme A ）。在赖氨酸（Lysine）、羟赖氨酸(hydroxylysine)和色氨酸(Tryptophan)的代谢过程中，戊二酰辅酶A（glutaryl coenzyme A）经戊二酰辅酶 A 脱氢酶 (GCDH) 催化氧化为巴豆酰辅酶A（S-but-2-enoylcoenzyme A ）。在调控过程中，CDYL（CDY-like）作为一种巴豆酰辅酶A水合酶，可将巴豆酰辅酶 A 转化为β-羟基丁酰辅酶 A，并负调节组蛋白Kcr。 巴豆酰化修饰
使用：乳酸是葡萄糖不完全氧化的产物。研究表明，体外培养条件下95％的葡萄糖转变为乳酸，这降低了营养物质的代谢效率，降低培养基pH值，增加渗透压。在氧气供给不足的情况下，NADH转运系统苹果酸－天冬氨酸穿梭系统活性低而不能将糖酵解产生的NADH氧化磷酸化为NAD+，细胞只得以降低能量需求的方式如激活乳酸脱氢酶将糖酵解产生的丙酮酸与NADH反应生产乳酸和NAD+，从而保证了糖酵解的顺利进行。另一个可能的解释是连接糖酵解与TCA循环的特异性酶如丙酮酸脱氢酶复合物、磷酸丙酮酸羧化酶激酶和丙酮酸羧化
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戊二酰辅酶A脱氢酶(GCDH)重组蛋白
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