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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 亚型:
Golden Syrian Hamster IgG
- 形态:
液体
- 保存条件:
The product shoμLd be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
- 克隆性:
Monoclonal, clone 37.51
- 标记物:
无
- 适应物种:
Mouse
- 保质期:
一年
- 库存:
现货
- 供应商:
乔默生物技术(上海)有限公司
- 应用范围:
FC, FA, IP Note: It is recommended that the reagent be titrated for optimal performance for each application.
- 浓度:
2 mg/mL
- 抗体英文名:
anti-Mouse CD28 Purified,37.51 Clone,Functional assay
- 抗体名:
CD28
- 规格:
500 μg
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文献和实验分钟收集细胞; 5. 将上一步离心得到的细胞沉淀,重悬至扩增培养基中,并进行计数。 1.3 细胞刺激(次日) 扩增培养基成分:RPMI1640 + 10% 血清 + 5ug/ml anti-mouse CD28(克隆号:37.51) + 双抗 1. 将细胞浓度调整至 1-2×10^6/ml(本次实验为 2×10^6/ml,该浓度偏大); 2. 从 4°C 冰箱取出前一天包被的板子,弃掉包被液,无菌PBS洗涤3遍; 3. 每孔加入 500μl 细胞悬液,放置于 37°C 含 5% CO2 细胞
Anti-factor Xa (Anti-Xa) Assay
The anti-factor Xa (anti-Xa) assay is a functional assay that facilitates the measurement of antithrombin (AT)-catalyzed inhibition of factor Xa by unfractionated heparin (UFH) and direct inhibition of factor Xa by low-molecular-weight
A Protocol for Heterologous Expression and Functional Assay for Mouse Pheromone Receptors
us to functionally express V2Rs on the surface of heterologous cells. In this chapter we describe protocols for maintenance and expansion of the R24 cell line and functional assays for V2Rs using these cells.
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