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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
储存-20℃,干燥、避光、密封
- 保质期:
2年
- 英文名:
Karavilagenin B
- 库存:
1000
- 供应商:
四川省维克奇生物科技有限公司
- CAS号:
912329-02-3
- 规格:
2mg 现货
WKQ-0025855
英文名称:Karavilagenin B
分子式:C31H52O3
分子量:472.74
CAS:912329-02-3
纯度:HPLC≥97%
沸点:552.4±40.0 °C | Condition: Press: 760 Torr
酸度系数:14.54±0.29 | Condition: Most Acidic Temp: 25 °C
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文献和实验, Cas9 mediates cleavage of target DNA upstream of PAM to create a DSB within the protospacer. ( b ) Design of chimeric sgRNA for genome cleavage. The SpCas9 nuclease ( yellow ) binds to genomic DNAdirected by the sgRNA consisting of a 20-nt guide
.Further trypan blue staining assay showed that the percentage of survival cells also decreased in a dosedependent manner following treatment with clivorine but not with monocrotaline (Fig. 2(B)). This suggests that clivorine inhibits human normal liver L-02 cells
设计了 sgRNA 之后怎么办呢?还是这篇文章先讲一下这里的框架首先是实验设计Experimental design1. Target selection for sgRNA--选择靶点详情见:CRISPR-Cas9 基因敲除 sgRNA 设计 https://www.jianshu.com/p/ 8222f449cb442. Approaches for sgRNA construction and delivery.sgRNA 的合成和投递有两种方式:A)PCR;B)单独的 sgRNA
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