Blue PMMA Particles-COOH, 600

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

?wide scale in a single experiment. The protocols described in this unit are based on separation of protein complexes by blue native polyacrylamide electrophoresis (BN?PAGE), the most versatile native gel system, and the closely related milder

Phagocytosis

and 2 x in PB.     Adjust the concentration to of particles to 109 particles/ml. Can now be frozen at -20 ° C.     For labeling, resuspend the pellet of 2 x 1010 particles in 20 ml Na2 HPO4 (50 mM, pH 9.2)     Add

细胞生长检测6：AlamarBlueTM摄入法

AlamarBlueTM 摄入法 1、按MTT检测法培养细胞和用不同稀释度的细胞因子处理细胞。2、在培养结束前24小时加入Alamar Blue染料，96孔细胞培养板每孔20μl。3、在培养结束后，直接在酶联检测仪上测定光吸收值。氧化型Alamar Blue的最大光吸收在570nm，用OD570 减去OD600 即为活细胞还原的Alamar Blue染料，颜色深浅与活细胞数成正比。