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2-8℃
- 保质期:
6个月
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100
- 供应商:
百欧泰
- 规格:
10 ml
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文献和实验Evaluating Modulators of “Regulator of G‐protein Signaling” (RGS) Proteins
?protein Signaling? (RGS) proteins constitute a class of intracellular signaling regulators that accelerate GTP hydrolysis by heterotrimeric G? subunits. In recent years, RGS proteins have emerged as potential drug targets for modulation by small molecules
Antibody Precipitation with Protein A or G
#20333) or ImmunoPure® Immobilized Protein G (Pierce product #20398). Primary antibody Immunoprecipitation buffer: 20 mM sodium phosphate, pH 7.5, 500 mM NaCl, 0.1% SDS, 1% NP40, 0.5% sodium deoxycholate and 0.02% sodium
Protein Syntheses in Cell Free Systems
such that the following are added to yield final concentration: ATP to 1.0 mM ATP GTP to 0.1 mM GTP Creatine phosphate to 10 mM Creatine kinase to 160 µg/ml Amino acids to 40 µm each Incubate the mixture for 45
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