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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 保质期:
6个月
- 库存:
100
- 供应商:
百欧泰
- 规格:
10 ml/20 ml
| 规格: | 10 ml | 产品价格: | ¥950.0 |
|---|---|---|---|
| 规格: | 20 ml | 产品价格: | ¥1500.0 |
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
EGTA stock, pH 8.0) in 100 ml PBS, pH 7.2-7.4 Heat about 80ml H2 O to 600 C and add paraformaldehyde; add 1-2 drops of 10M NaOH to get paraformaldehyde in solution. Cool to room temperature, add 10 ml 10X PBS, adjust pH with HCl, add MgCl
Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)
be much trouble. Move to 100x, focus, then go to bright field. Now move the condenser slightly away from the bright field position until the image appears three-dimensional. This works with condensers that have turrets that rotate for different modes of viewing
for 5 minutes(16,000xg,4 ℃ )and discard the supernatant. 5.Wash with lysis buffer,by centrifuging 5 minutes (16,000xg,4 ℃ ),repeat wash twice. C: The crosslinking of ganglioside 1.Add 200-400 µl 50-100 uM ganglioside (diluted in PBS from 5mM in DMSO stocking
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