- ￥950 - 1500
- 百欧泰
- BPMM-1000-COOH
- 2025年11月18日
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- 保存条件：
2-8℃
- 保质期：
6个月
- 库存：
100
- 供应商：
百欧泰
- 规格：
10 ml/20 ml
| 规格： | 10 ml | 产品价格： | ￥950.0 |
|---|---|---|---|
| 规格： | 20 ml | 产品价格： | ￥1500.0 |
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
) Dissect embryos or small pieces of tissue (e.g. the size of a retina from an adult rat works well with this procedure) into PBS containing 2mM MgCl2 (PBS + Mg2+) on ice. PBS(10X) 80 g NaCl 2 g KCl 11.5g Na2HPO4 2 g KH2PO
solution)to microcentrifuge tube. 2.Add 2.5x 10,000 particles of 1 uM Fluosphere beads. 3.Mix overnight at 4 ℃ with 200 µl 5mg/ml 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. 4.Wash with PBS,by centrifuging,repeat wash 3 times. 5.Resuspend the bead
Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)
gently for 10 min. then cooled to room temperature before use. Prepare a vaseline mount of the yeast-Paramecium mix and begin observing in dark field, starting with the lowest power. Paramecium move rather quickly, thus that they can be difficult
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