qPCRBIO SyGreen® 一步法试剂盒 Go Hi-

特点​

1. 热稳定性逆转录酶，作用温度范围45°C至55°C

2. 先进的RNase抑制剂

3. 无PCR抑制效应的嵌合染料

4. 快速的延伸速率，有助于获得更早的Ct值

5. 业界领先的灵敏度

6. 更高的检测限

7. 抗体介导的热启动PCR

8. 兼容所有标准和快速循环的实时荧光定量PCR平台

​应用​

1. 相对基因表达分析

2. 绝对定量

3. 极低拷贝数靶标的检测

产品描述​

qPCRBIO SyGreen 一步法试剂盒专为在单管中进行快速、高特异性、超灵敏的cDNA合成及实时荧光定量PCR而设计。

您可选择：

• ​qPCRBIO SyGreen® 一步法 Detect​：用于检测极低拷贝数的靶标。

• ​qPCRBIO SyGreen® 一步法 Go​：用于从高浓度RNA模板中获得快速而准确的结果。

​图1. qPCRBIO SyGreen 一步法 Go 与竞争产品 Bioline 的比较​

结果说明：使用来自小鼠肝脏的总RNA（浓度梯度为每20μl反应25pg至250ng）扩增ACTG1基因。qPCRBIO SyGreen 一步法 Go在高质量RNA浓度下表现相当，在低RNA浓度下性能更优：显示出线性间隔良好的扩增曲线、扩增提前3-4个循环，且引物二聚体形成更少。

​Comparison of qPCRBIO SyGreen 1-Step Go (purple) against competitor Bioline (blue): Shows amplification traces of the ACTG1 gene from a dilution series of total RNA extracted from mouse liver. Total RNA concentration varied from 25pg to 250ng per 20μl reaction. Cycling conditions were 45°C 10 minutes for cDNA synthesis, followed by 95°C 2 minutes hot start, then 45 cycles of 95°C 10sec, 60°C 10sec on Roche LC480. qPCRBIO SyGreen 1-Step Go had equal performance at high RNA concentrations and superior performance at lower RNA concentrations, displaying linear spacing between amplification curves, earlier amplification by 3–4 cycles, and lower prevalence of primer dimer.

图2. qPCRBIO SyGreen 一步法 Detect 与 qPCRBIO SyGreen 一步法 Go 的比较​

结果说明：展示了各产品理想的模板适用范围。qPCRBIO SyGreen 一步法 Go 可有效扩增每反应100pg至100ng范围的总RNA。qPCRBIO SyGreen 一步法 Detect 可有效扩增每反应10pg至10ng范围的总RNA（图a），并显示了熔解曲线分析结果（图b）。

Comparison of qPCRBIO SyGreen 1-Step Detect (blue) and qPCRBIO SyGreen 1-Step Go (purple), showing ideal template ranges for each product: The ACTG1 gene was amplified from a dilution series of total RNA extracted from mouse liver. qPCRBIO SyGreen 1-Step Go (purple) shows efficient amplification of total RNA in the range 100pg to 100ng per reaction. qPCRBIO SyGreen 1-Step Detect (blue) shows efficient amplification of total RNA in the range 10pg to 10ng per reaction (figure a). Cycling conditions were 45°C 10min for cDNA synthesis, followed by 95°C 2min hot start, then 45 cycles of 95°C 10sec, 60°C 30sec, concluding with a melt analysis (figure b) on a Roche LC480.