- ￥2500
- 百欧泰
- BYMM-8000-Avidin
- 2025年11月17日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8℃
- 保质期：
6个月
- 库存：
100
- 供应商：
百欧泰
- 规格：
10 ml
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文献和实验CELL ADHESION Procedure: 1) Dissolve/dilute coating substrate in ddH2O at 4 C. A common working dilution for the laminin-1 positive control and BSA (Sigma A8412) negative control is 40 ug/ml. For SN-peptide, use 20 uM (MW of SN
- make a 5 uM stock solution of adaptors - add 5 ul of the top strand adaptor (100 uM) and 5 ul of the bottom strand adaptor (100 uM) to 80 ul H2O + 10 ul 10X BRL buffer 3. c) heat both to boiling, and let cool to RT. Ligation of Adaptors
ELISPOT (Enzyme-linked ImmunoSPOT)
cytokine. The detected cytokine is then visualized using avidin-HRP, and a precipitating substrate (e.g., AEC). The colored end product (spot) represents an individual cytokine-producing cell. The spots can be counted manually (e.g., with a dissecting
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