- ￥2500
- 百欧泰
- BYMM-3000-Avidin
- 2025年11月17日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8℃
- 保质期：
6个月
- 库存：
100
- 供应商：
百欧泰
- 规格：
10 ml
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文献和实验CELL ADHESION Procedure: 1) Dissolve/dilute coating substrate in ddH2O at 4 C. A common working dilution for the laminin-1 positive control and BSA (Sigma A8412) negative control is 40 ug/ml. For SN-peptide, use 20 uM (MW of SN
treatments or different mutants you might have to adapt the seeding density. It is important that all the cultures are confluent at the start of the experiment. 2. Using a (yellow) pipette tip make a straight scratch, simulating a wound. Often we make
solution)to microcentrifuge tube. 2.Add 2.5x 10,000 particles of 1 uM Fluosphere beads. 3.Mix overnight at 4 ℃ with 200 µl 5mg/ml 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. 4.Wash with PBS,by centrifuging,repeat wash 3 times. 5.Resuspend the bead
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