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文献和实验(radioactive/fluorescent/enzyme) ssDNA. This is also known as hybridization. Process relies on the ssDNA (probe) hybridizing (annealing) to the DNA on the membrane due to the binding of complementary strands. Probing is often done with 32-P labeled ATP, biotin
PRINS for the Detection of Unique Sequences
polymerase antibody, and stringent washing, we localized the human SRY gene to Yp11.31-p11.32 in chromosome preparations in situ . Locus-specific oligonucleotide probes (i.e., PRINS primers) were annealed to chromosomal DNA fixed on glass slides and extended
PRINS for Mapping Single-Copy Genes
, the human SRY gene was localized to Yp11.31-p11.32 and the SOX3 gene to Xq26-q27. In other studies, we localized specific deletions of RBM and DAZ , candidate genes for AZF (azoospermia factor) to proximal Yq11.2, the AZF region, in an infertile male. Locus
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