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文献和实验yzzaici888 大家好,本人最近一直在做p-p38的磷酸化,但是一直都做不好。希望各位大侠帮助解决: 蛋白上样量80-100ug,半干转膜70min,5%BSA封闭70min,一抗CST(#9211) 1:500加,4℃孵育过夜,二抗1:8000加室温70min。曝光出的结果很不好,杂带很多,也分析不出那条是目的条带,且没有趋势,见下图 15001046419
(radioactive/fluorescent/enzyme) ssDNA. This is also known as hybridization. Process relies on the ssDNA (probe) hybridizing (annealing) to the DNA on the membrane due to the binding of complementary strands. Probing is often done with 32-P labeled ATP, biotin
PRINS for the Detection of Unique Sequences
polymerase antibody, and stringent washing, we localized the human SRY gene to Yp11.31-p11.32 in chromosome preparations in situ . Locus-specific oligonucleotide probes (i.e., PRINS primers) were annealed to chromosomal DNA fixed on glass slides and extended
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