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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
柏莱源(天津)生物科技有限公司
- 库存:
现货
- 靶点:
CD11b
- 级别:
详询
- 目录编号:
详询
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
自收货起一年
- 抗体英文名:
APC anti-mouse/human CD11b Antibody
- 抗体名:
APC 抗小鼠/人 CD11b 抗体
- 标记物:
详询
- 宿主:
大鼠
- 适应物种:
小鼠, 人类, 食蟹猴, 恒河猴
- 免疫原:
转染小鼠 CXCR6 的细胞
- 亚型:
Rat IgG2b, κ
- 形态:
液体
- 应用范围:
FC
- 保存条件:
2至8度储存,避光
- 浓度:
0.2 mg/mL
- 规格:
询价
CD11b是一种170 kDa的糖蛋白,亦被称为αM整合素、Mac-1α亚基、Mol、CR3及Ly-40。作为整合素家族成员,CD11b主要表达于粒细胞、单核细胞/巨噬细胞、树突状细胞、自然杀伤(NK)细胞以及部分T细胞与B细胞亚群。该蛋白通过与CD18(β2整合素)非共价结合形成Mac-1复合体。Mac-1通过与其配体ICAM-1(CD54)、ICAM-2(CD102)、ICAM-4(CD242)、iC3b和纤维蛋白原结合,在细胞间相互作用中发挥重要功能。
| 产品名称 | APC 大鼠 IgG2b, κ 同型对照 |
| 已验证反应性 | 小鼠, 人, 食蟹猴, 恒河猴 |
| 报道反应性 | 黑猩猩, 狒狒, 兔 |
| 抗体类型 | 单克隆抗体 |
| 宿主物种 | 大鼠 |
| 免疫原 | C57BL/10 小鼠脾细胞 |
| 制剂 | 磷酸盐缓冲液 (pH 7.2),含 0.09% sodium azide |
| 制备方式 | 抗体通过亲和层析纯化,并在最佳条件下与APC偶联。 |
| 浓度 | 0.2 mg/ml |
| 储存与处理 | 抗体溶液应原液保存在 2°C 至 8°C 之间,避免长时间暴露在光线下,请勿冷冻。 |
| 应用 | 流式细胞术 (FC) - 经过质控验证 |
| 建议用量 | 每批抗体均通过免疫荧光染色和流式细胞分析进行质控检测。对于流式细胞术染色,建议在100微升体积中,每百万细胞使用该试剂≤0.25微克。建议为每次应用进行滴定以确定最佳使用量。 |
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文献和实验[1]Guo H, Cooper S, Friedman AD. In Vivo Deletion of the Cebpa +37 kb Enhancer Markedly Reduces Cebpa mRNA in Myeloid Progenitors but Not in Non-Hematopoietic Tissues to Impair Granulopoiesis. PLoS One. 2016 Mar 3;11(3):e0150809. doi: 10.1371/journal.pone.0150809. PMID: 26937964; PMCID: PMC4777376.
[2]Chen Q, Liu Y, Jeong HW, Stehling M, Dinh VV, Zhou B, Adams RH. Apelin+ Endothelial Niche Cells Control Hematopoiesis and Mediate Vascular Regeneration after Myeloablative Injury. Cell Stem Cell. 2019 Dec 5;25(6):768-783.e6. doi: 10.1016/j.stem.2019.10.006. Epub 2019 Nov 21. PMID: 31761723; PMCID: PMC6900750.
[3]Frieler RA, Chung Y, Ahlers CG, Gheordunescu G, Song J, Vigil TM, Shah YM, Mortensen RM. Genetic neutrophil deficiency ameliorates cerebral ischemia-reperfusion injury. Exp Neurol. 2017 Dec;298(Pt A):104-111. doi: 10.1016/j.expneurol.2017.08.016. Epub 2017 Aug 31. PMID: 28865993; PMCID: PMC5658240.
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