新绿原酸 906-33-2 苯丙素类

本品为分析标准品。

使用本产品的案例（仅供参考）

HPLC

About 5 µL of the diluted AVTE sample solution was analyzed using an HPLC System and separated using an C18 column (2.6 µm, 4.6 × 150 mm). Mobile phase A was water containing 0.5% acetic acid, and mobile phase B was acetonitrile. The flow rate was 0.5 mL/min with a column temperature of 30℃, and the detection wavelength was 328 nm. The gradient elution conditions were as follows: Equilibrium stage was set for 10 min with 12% B (isocratic) then, 0–30 min with 12%–45% B (linear gradient), 30–35 min with 45%–100% B (linear gradient), and 35–40 min with 100% B (isocratic).

来源文献：Li C, Tan F, Yang J, Yang Y, Gou Y, Li S, Zhao X. Antioxidant Effects of Apocynum venetum Tea Extracts on d-Galactose-Induced Aging Model in Mice. Antioxidants (Basel). 2019 Sep 8;8(9):381. doi: 10.3390/antiox8090381. PMID: 31500342; PMCID: PMC6770887.

UHPLC

Chromatographic separation of SPLFA and SPLF was done on an Agilent 1290 UHPLC system  coupled to an auto sampler, a diode array detector (DAD) and an ACQUITY UHPLC® HSS T3 column (2.1 ×100 mm, 1.8 μm; Waters, Milford, MA, USA)maintained at 30 ◦C. Eluent A was 0.05% formic acid in water and eluent B was 0.05% formic acid in acetonitrile. Elution of the SPLPA compounds was achieved using the following linear gradient elution (in %B): 0 min, 5%; 20 min, 60%; 20.1 min, 5%; 30 min, 5%, and for the SPLF compounds: 0 min, 20%, 25 min, 50%, 25.1 min, 20%, 35 min, 20%. The injection volume was 5 μL, with a flow rate of 0.3 mL/ min. All sample solutions were filtered through a 0.22 μm PTFE filter (Agilent Technologies) before analysis. The detection wavelength was 320 nm for SPLPA and 254 nm for SPLF. 

来源文献：[1] Luo D , Mu T , Sun H .Profiling of phenolic acids and flavonoids in sweet potato (Ipomoea batatas L.) leaves and evaluation of their anti-oxidant and hypoglycemic activities[J].Food Bioscience, 2020, 39(44):100801.DOI:10.1016/j.fbio.2020.100801.

HPLC

The phenolic acid contents were also measured usingHigh Performance Liquid Chromatography.Chromatographic separation was conductedwith an C18 column (4.6 mm × 250 mm,5 mm) and a binary solvent system of (A) methanol (0.1%H3PO4) and (B) water (0.1% H3PO4). The elution wasperformed at a flow rate of 1.0 mL/min and the columntemperature was maintained at 30℃. The contents of phenolicacids [CGA, neochlorogenic acid (NCGA), cryptochlorogenicacid (CCGA), p-coumaryl quinic acid, and caffeoyl shikimicacid] were detected at 320 nm and the contents of anthocyanin(cyanidin-3-O-glucoside chloride and cyanidin 3-O-rutinosidechloride) were quantified at 525 nm.

来源文献：Su Z, Jia H, Sun M, Cai Z, Shen Z, Zhao B, Li J, Ma R, Yu M, Yan J. Integrative analysis of the metabolome and transcriptome reveals the molecular mechanism of chlorogenic acid synthesis in peach fruit. Front Nutr. 2022 Jul 19;9:961626. doi: 10.3389/fnut.2022.961626. PMID: 35928835; PMCID: PMC9344011.