2-Pyridin-4-ylbenzoic acid;133

Clonality - X Chromosome Inactivation Assay

) 10X TBE (1 M Tris, 0.9 M Boric Acid, and 0.01 M EDTA) (Life Technologies) Loading dye (95% formamide, 20 mM EDTA, 0.05% bromophenol blue, 0.05% xylene cyanol) 2. Methods TIP: Investigators must be especially careful when using this methodology

How to isolate mRNA(分离mRNA)

while retaining the oligo-dT bound poly（A）-RNA. After several washing steps under conditions less stringent than for annealing, poly（A）-RNA is eluted in water or low salt Tris buffer. Elution volumes vary from 20-250 μl for most kits accepting up to 1 mg of total

Preparation of Carboxypeptidase Y and Its Properties

】 Carboxypeptidase Y is a kind of proteases in bread yeast. It has the ability to remove most amino acid residue, including that of proline, from the C-termini of proteins and peptides. These unique properties render a useful tool in C-termini