N-(4-(2,3-DIOXOPROPYL)PHENYL)F

DNA Sequencing

(Pharmacia) Stop solution (95% deionized formamide, 20mM EDTA, pH 7.5, 0.1% each of bromophenol blue and xylene cyanol FF) 1) Denature dsDNA by the addition of 8ul DNA (approximately 3ug) to a sterile microcentrifuge tube containing 2ul freshly made 2M

PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)

） from related herpesviruses. The reverse primer sequence was as follows: TCGAATTCNCCYAAYTGNCCNT where Y = T + C, and N = A + G + C + T, and the 8-base 5'-terminal extension comprises a EcoRI site （underlined） and flanking spacer to ensure the restriction

FISH

. Meanwhile in Coplin jars: 9.  Hydrate:  2x SSC x2-5 min. 10. (Optional, to reduce cytoplasm bkgd: 10 mg/mL RNAse (DNAse-free) in 2x SSC, 37ºC x30 min.) 11. Denature in 2x SSC + Formamide (50% vol), 72ºC x2-5 min. 12. Dehydrate:  Ice cold 70% EtOH, 85% EtOH, 95