- ￥290
- 源叶
- 中国
- Y92415-100mg
- 2025年10月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
RT
- 英文名：
7-BROMO-2-METHYLBENZOTHIAZOLE
- 库存：
99
- 供应商：
上海源叶生物科技有限公司
- CAS号：
110704-32-0
- 规格：
100mg
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文献和实验【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
aliquotsat -20°C. 5. Lysis buffer: mix 4.6 mL of STE, 100 mL of 0.5 M EDTA,and 200 mL of 10% sodium dodecyl sulfate (SDS). Add100 mL of 10 mg/mL proteinase K and 20 mL of 2 mg/mLRNase A prior to use (see Note 1). 6. Saturated phenol (pH
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
) can be added in conjunction with NBT to increase the reaction rate further. Prepare a 100X stock of 2 mg/ml in H2O and use immediately. PMS is very unstable. To aid in identification of stained cells, the tissue can be processed for cryostat sections
DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver Nitrate
3-3.5 volumes of 100% ethanol, 1/10 volumes sodium acetate (pH 5.2) and 5-10µl glycogen (10 mg/ml) used as a carrier, followed by incubation at -70¡ãC for 30 min to precipitate DNA. After Centrifugation at 12000-¡Á g for 10 minutes, the supernatant was removed
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