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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 英文名:
1-BOC-4-METHYLPIPERIDINE
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
123387-50-8
- 规格:
250mg
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文献和实验PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
; the mismatch positions were used as 4-base degeneracies for the primers (shown as stars; 5 in F and 4 in R), as shown above. Despite their degeneracy, the primers could be used to amplify a 250 bp sequence from viruses differing in sequence by as much as 50
in water gradient, both containing 0.1% (v/v) TFA, at 1 mL/min over 30 minutes at 60 °C with a C18, 5 μm, 50 X 2 mm column) and MALDI (Fig. 5). Purification with reverse phase HPLC on a Vydac C18 column (10 μm, 22 mm x 250 mm) proceeded, using
Microarray Hybridization Protocol
on it. Therefore, the first thing to do is to wash them.) Place bottle of Microhyb solution (Research Genetics HYB250.GF) at 65° C at least 15 minutes before use. Denature 1 ml of tRNA (50 ?g/?l) (Sigma R-8759) at 95 ?C for 10 minutes.
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