3-Bromo-5-cyanobenzyl bromide;

James Hardwick CNBr Cleavage Procedure

with a screen to see a strong signal.   Gel Recipes: Tris Anode (lower) Buffer 1 liter     0.2 M Tris pH 8.9 24.2 g Tris base   Bring to 1 liter with water--adjust to pH

Fast and reliable mini-prep RNA extraction from Neurospora crassa

containing high levels of nucleases. Extraction from starved cultures or plate mycelia with the LiCl method (Chambers and Russo, Fungal Genet. Newsl. 1987. 33:22-24) gave completely degraded RNA as judged by gel electrophoresis. In trying to improve

DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver Nitrate

or 5% polyacrylamide gels and stained in ethidium bromide for visualization (1).  The samples were diluted 1:9 in loading buffer (95% formamide, 10mM NaOH, 0.05% bromophenolblue, and 0.05% xylene cyanol), heated at 95˚C for 5 min, and quickly plunged into ice, then 5µl