- ￥1540
- 源叶
- 中国
- Y86735-100mg
- 2025年10月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8℃
- 英文名：
benzo[d]oxazol-7-amine
- 库存：
99
- 供应商：
上海源叶生物科技有限公司
- CAS号：
136992-95-5
- 规格：
100mg
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文献和实验A safe method of extracting DNA from Coccidioides immitis
, the whole container is transferred to an autoclave and steamed at 1 atm (100 C) for 15 min. This raises the temperature of the culture to about 95 C (as measured in trials with a thermocouple). The culture is allowed to cool, 1 ml is plated on 2X GYE or potato
Single tube confirmation PCR protocol
containing 60 U/ml of Zymolyase (This solution is generated by resuspending 6 mg of 100T Zymolyase in 10 mls of water. Ordering information: Seikagaku Corp., #120493-1). - Sterile yellow tips work great for this task. - Repeat this step
DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver Nitrate
3-3.5 volumes of 100% ethanol, 1/10 volumes sodium acetate (pH 5.2) and 5-10µl glycogen (10 mg/ml) used as a carrier, followed by incubation at -70¡ãC for 30 min to precipitate DNA. After Centrifugation at 12000-¡Á g for 10 minutes, the supernatant was removed
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