(7S)-6,7-二氢-5H-7-羟基 –环戊[B]并吡啶;

【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离

for one reaction: The primary PCR cycling conditions are as follows: (a) 95°C for 1 min 30 s (b) 10 cycles of ●● 95°C for 5 s ●● 70°C for 3 min 10 s with decreasing 0.5°C each cycle(c) 20 more cycles of ●● 95°C for 5 s ●● 65°C

Crystallization of Kinesin Family Motor Proteins

) PEG 4000, 2.5 mM ATP, 10 mM MgCl2Reservoir: 100 mM Na-acetate pH 4.6, 150 mM KCl, 7% PEG 4000, 5 mM ATP, 20 mM MgCl2Kull et al., 1996Ncd 335-700Sitting drop Room temperature7 mg/ml protein in 10 mM Pipes, pH 7.2, 100 mM NaCl, 1 mM EGTA, 1 mM DTT, 7% (w

v468 Chapter 2 Wnt活性蛋白分离及应用

: L-Wnt3A (ATCC , Manassas, VA; ATCC# CRL-2647) (see Note 1 ). 2. Cell culture medium: Dulbecco’s minimum essential medium(DMEM) supplemented with 10% (v/v) fetal bovine serum(FBS), 1:100 dilution of penicillin–streptomycin solution with10,000 U