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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
1219967-55-1
- 规格:
100mg
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文献和实验DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
diiferent blades for each part of the tumor. Overlay with 20 ul mineral oil. Close cap and seal with parafilm. DIGESTION: Incubate overnight for shaking at 120 rpm, 55 C. Day 2: Add 0.3 ul fresh conc Proteinase K (20 mg/ml stock
A safe method of extracting DNA from Coccidioides immitis
, the whole container is transferred to an autoclave and steamed at 1 atm (100 C) for 15 min. This raises the temperature of the culture to about 95 C (as measured in trials with a thermocouple). The culture is allowed to cool, 1 ml is plated on 2X GYE or potato
(至少室温放置30min)无菌的40% detrose贮液。 (3)YPAD(斜面)培养基(可用于保菌或培养营养缺陷菌株,100mL) 在YPD液体培养基中加入硫酸腺嘌呤(Adenine hemisulfate salt,腺嘌呤半硫酸盐)40mg,琼脂(Bacto-agar)(液体培养基不加)2g。加ddH2O至90mL,调pH值至5.8(液体调至4左右),定容至95mL,高温灭菌(121℃,15min)。待温度降至55℃时,加入5mL预热(至少室温放置30min)无菌的40
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