- ￥1700
- 源叶
- 中国
- Y69442-250mg
- 2025年10月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8℃
- 英文名：
5-BROMO-2-METHYL-4-PHENYL-1,3-OXAZOLE
- 库存：
99
- 供应商：
上海源叶生物科技有限公司
- CAS号：
20662-93-5
- 规格：
250mg
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文献和实验filter sterililize; no DEPC treatment. Preparation of dNTP's. The quick protocol for ~100mM stock. dilute all four dNTP's (250mg of each) in 3.676ml of H2 O; + 424µl 5M NaOH; check the pH: ~0.5µl on pH-paper
of fixative.): Lidocaine 50 mg per mL, dissolved 95% EtOH (warm to dissolve). Add slowly to perfusion solution with stirring to make final lidocaine solution concentration wanted (e.g. 1mg/mL = 0.1%) Note: Do not add the lidocaine directly
Methods for use with the mTn-3xHA/lacZ-mutagenized library
was made from a circle-zero strain (also rho-minus) so this is not necessary. Screening 30, 000 transformants should give you 95% coverage of the yeast genome. To minimize double integrants, transformations should contain the lowest amount of DNA practicable
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