((2,5-DIMETHYLPHENYL)SULFONYL)

Crystallization of Kinesin Family Motor Proteins

protein in 20 mM HEPES pH 7.5, 100 mM NaCl, 1 mM EGTA, 0.7 M Li2S04, 2 mM ADP, 10 mM MgCl2Reservoir: 20 mM HEPES pH 7.5, 1.4 M Li2SO4, 1 mM EGTA, 1 mM DTT, 10 mM MgCl2Sablin et al., 1998Ncd 295-700Hanging drop Room temperature5 mg/ml protein in 25 mM Na2PO

Identifying Functional Sites Based on Prediction of Charged Group Behavior

Figure 8.6.1 Alanine racemase tyrosines. Predicted titration curves (ensemble average charge C as a function of the pH) for tyrosine residues Y225 (solid squares), Y239 (hollow circles), Y265 (solid triangles), Y269 (hollow triangles), and Y284

酵母分批培养实验操作方法

℃。 5. 接种、发酵： 适当降低通风量，使高压接近零。在接种口(进料口)周围放置浸有酒精的棉花，点燃后迅速打开接种口，将菌种加入到发酵罐中，旋紧接种口盖子，熄灭火焰，调节通风量，接上玻璃转子流量计，使流量计读数在8.0左右，同时保持罐压0.04Mpa，保温保压发酵48hr 6. 取样 培养基放入罐前取50ml，测定其还原糖，菌体量。发酵每隔4h后取样100 m测定菌体量、总还原糖。取样时，将取样口最初的10ml培养液弃掉。 具体