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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
\N
- 英文名:
4H-Pyrazolo[4,3-c]pyridin-4-one,1,5-dihydro-
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
41373-13-1
- 规格:
100mg
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文献和实验【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
aliquotsat -20°C. 5. Lysis buffer: mix 4.6 mL of STE, 100 mL of 0.5 M EDTA,and 200 mL of 10% sodium dodecyl sulfate (SDS). Add100 mL of 10 mg/mL proteinase K and 20 mL of 2 mg/mLRNase A prior to use (see Note 1). 6. Saturated phenol (pH
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
a homogeneous wetting of the surface by the poly-L-lysine solution in Step 5. 3. Wash for 2 h in running tap water. Rinse twice in H2 O. 4. Dip slides twice in 95% ethanol. Air-dry. 5. Dip slides into the poly-L-lysine
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