2，2'，7，7'-四(吡啶-4-基)-9，9'-螺双[芴]

Single Primer ("Semi-Random") P

Reamplification 40 µL H2O 10 µL 10x M-PCRB 10 µL 4 dNTPs @ 2 mM each 10 µL Pn primer @ 5 µM 10 µL Po primer @ 0.2 µM 10 µL DNA from part 1 10 µL "T/TS" @ 0.4 u Taq/µL --------- 100 µL Load glass capillaries and amplify: 30"x94°C 30

AP Assay and FGF Binding

: 5’TCTGTTGTGTTTCCTCCCTGTTGG3’ Amplify 360 bp of hGH sequence present in 3’ UTR of the transgene. PCR amplification conditions... 27 cycles of 94°C x 60 seconds, 55°C x 60 seconds and 72°C x 90 seconds.  

盐溶液配制

/ml) 18o C 1.0465 1.0913 1.1351 1.1772 1.2179 1.2571 1.2946 1.3309 (AcO)2 Mg Magnesium Acetate, Mg(C2 H3 O2 )2 x 4H2 O; Mw=214.46g/m; (store at NT). Conc. Stock 50ml 100ml 300ml Mg(C2 H3