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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C
- 英文名:
2-Butenedioic acid (2Z)-, 1,4-bis(1-methylethyl) ester
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
10099-70-4
- 规格:
250mg
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文献和实验of the supernatant is transfered to a Robbins PCR reaction tube (in 96 well tube format) and precipitated with 200 ul of 95% ethanol, washed three times with 70% ethanol, and stored dry at -20degC for future use. The following is an automated, 96 well, double
boil for 15 min spin and take supernatant (containing the DNA) into fresh tube precipitate with 10 µl 3 M NaAcetate, 5 µl glycogen (10 mg/ml) , 400 µl 100 % Ethanol incubate at -70 °C for at least 30 min spin, wash
Clonality - X Chromosome Inactivation Assay
(1:1:1, v:v:v) (Gibco) 3 M Na Acetate, pH 5.2 (Life Technologies) Isopropanol Glycogen, 10 mg/ml (GenHunter) 70% ethanol Buffer 1 (50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 50 mM NaCl) Hha I restriction enzyme (Life Technologies) REact 2 Buffer
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