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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
避光,-20°C,充氩
- 英文名:
Cy3 NHS ester
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
1393363-07-9
- 规格:
1mg
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文献和实验-BSA was prepared using an Alexa Fluor 488 protein labeling kit from Molecular Probes. Alexa488-BSA-Dig was prepared by labeling Alexa488-BSA with 3-amino-3-deoxydigoxigenin hemisuccinamide, succinimidyl ester (Molecular Probes). Cy3-BSA and Cy5-BSA
Revised amino-allyl labeling protocol
C indefinitely. D. Coupling Add 0.5 m L 1M sodium bicarbonate, pH 9.0 to 50 mM final. Check 1M stock solution periodically for fluctuations in pH. Monofunctional NHS-ester Cy3 (PA23001) and Cy5 dye (PA25001, Amersham) is supplied
Protocol for Reverse Transcription and Amino-allyl Coupling of RNA
in pH. Monofunctional NHS-ester Cy3 (PA23001) and Cy5 dye (PA25001, Amersham) is supplied as a dry pellet. Each tube is sufficient to label 10 reactions under normal conditions. Dissolve dry pellet in 20 mL DMSO. Aliquot 2 mL into 10 single
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