(5-bromo-2-methylpyridin-3-yl)

Phycoerythrin conjugation protocol

, about 2.5 mg/ml PE R-Phycoerythrin, purified (Molecular Probes P-801 in 60% ammonium sulfate) PBS Phosphate buffered saline without Ca2+ /Mg2+ MeOH Methanol

Crystallization of Kinesin Family Motor Proteins

) PEG 4000, 2.5 mM ATP, 10 mM MgCl2Reservoir: 100 mM Na-acetate pH 4.6, 150 mM KCl, 7% PEG 4000, 5 mM ATP, 20 mM MgCl2Kull et al., 1996Ncd 335-700Sitting drop Room temperature7 mg/ml protein in 10 mM Pipes, pH 7.2, 100 mM NaCl, 1 mM EGTA, 1 mM DTT, 7% (w

免疫沉淀-蛋白印迹法

sepharose A beads were then added, and incubation continued for a further 2 hours at 4°C. [The Protein Sepharose A beads had previously been hydrated by leaving a solution of 100mg protein sepharose A and 15ml TBS (22.3g Tris, 8g NaCl, 1M HCl) on a shaking